06-284-AF488
Anti-Nitrotyrosine Antibody Alexa Fluor™ 488 Conjugate
from rabbit, ALEXA FLUOR™ 488
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Nitrotyrosine AlexaFluor™ 488 Conjugate
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biological source
rabbit
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
species reactivity (predicted by homology)
all
technique(s)
immunocytochemistry: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
nitration (Tyr)
Gene Information
human ... NOS1(4842)
General description
Nitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration. In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
Specificity
This rabbit polyclonal antibody detects tyrosine nitrated proteins.
Immunogen
Epitope: Nitrated Tyrosine
Nitrated Keyhole Limpet hemocyanain (KLH).
Application
Anti-Nitrotyrosine AlexaFluor 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
The unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
Quality
Evaluated by Immunocytochemistry in A431 cells treated with peroxytnitrite.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
Physical form
Affinity purified
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Certificates of Analysis (COA)
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