Anti-Cyclin A Antibody

The cell cycle is regulated by the interplay of many molecules. Key among these are the cyclins which are expressed and then degraded in a concerted fashion to drive the stages of the cell cycle. Cyclins combine with cyclin dependent kinases (cdks) to form activated kinases that phosphorylate targets leading to cell cycle regulation. A breakdown in the regulation of this cycle can lead to out of control growth and contribute to tumor formation. Defects in many of the molecules that regulate the cell cycle have been implicated in cancer. Cyclin A is a member of the Cyclin A family that can bind and activate Cdc2 and Cdk2 and is involved in the control of mitosis and S phase. During S phase, cyclin A forms a complex with Cdk2, the pRB-related protein p107 and E2F.

p58cyclin A; does not cross-react with cyclin B or cyclin D2, or 3

peptide corresponding amino acid residues 417-432 (C-KHGVSLLNPPETL-N-L)

Detect Cyclin A with Anti-Cyclin A Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP, WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

routinely evaluated by immunoblot on HeLa nuclear extract

58kDa

Format: Purified

Lyophilized

Protein G Chromatography

2 years at -20°C

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

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