06-1106
Anti-Sonic Hedgehog Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
sonic hedgehog, sonic hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila)
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SHH(6469)
General description
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.
Specificity
This antibody recognizes the N-terminus of Sonic Hedgehog.
Immunogen
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of Sonic HedgeHog.
Application
Anti-Sonic Hedgehog Antibody detects level of Sonic Hedgehog & has been published & validated for use in WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Sonic Hedgehog in colorectal carcinoma tissue.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Sonic Hedgehog in colorectal carcinoma tissue.
Quality
Evaluated by Western Blot in human fetal skeletal muscle tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.
Target description
~ 45 kDa
Linkage
Replaces: 04-971
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human fetal skeletal muscle tissue lysate
Human fetal skeletal muscle tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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