Skip to Content
Merck
CN

05-747

Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal

Upstate®, from rabbit

Synonym(s):

Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody, rabbit monoclonal, Upstate®, from rabbit

biological source

rabbit

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity

human, mouse, rat

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_002755
NM_030662

UniProt accession no.

Q02750

shipped in

dry ice

target post-translational modification

phosphorylation (pSer218/pSer222)

Quality Level

100

Gene Information

human ... MAP2K1(5604)

Analysis Note

routinely evaluated by immunoblot on RIPA lysates from PC-12 cells that have been treated with B-Raf

Application

Detect phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) using this Anti-phospho-MEK1 (Ser218/222)/MEK2 (Ser222/226) Antibody validated for use in WB.
Research Category
Signaling
Research Sub Category
MAP Kinases

Biochem/physiol Actions

phosphorylated MEK1/MEK2

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

45kDa

Immunogen

Peptide containing DpSMANpSF in which pS corresponds to phosphorylated amino acids 218 and 222 of human MEK1

Other Notes

Replaces: 04-1017

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A purified

Preparation Note

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Gonzalo L Gonzalez-Del Pino et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(36) (2021-09-03)
The RAF/MEK/ERK pathway is central to the control of cell physiology, and its dysregulation is associated with many cancers. Accordingly, the proteins constituting this pathway, including MEK1/2 (MEK), have been subject to intense drug discovery and development efforts. Allosteric MEK
Negative regulation of MAPKK by phosphorylation of a conserved serine residue equivalent to Ser212 of MEK1.
Gopalbhai, Kailesh, et al.
The Journal of Biological Chemistry, 278, 8118-8125 (2003)
Sigrun Polier et al.
Nature chemical biology, 9(5), 307-312 (2013-03-19)
Protein kinase clients are recruited to the Hsp90 molecular chaperone system via Cdc37, which simultaneously binds Hsp90 and kinases and regulates the Hsp90 chaperone cycle. Pharmacological inhibition of Hsp90 in vivo results in degradation of kinase clients, with a therapeutic
Dennis M Bjorklund et al.
Biomolecules, 12(7) (2022-07-28)
The kinome specific co-chaperone, CDC37 (cell division cycle 37), is responsible for delivering BRAF (B-Rapidly Accelerated Fibrosarcoma) to the Hsp90 (heat shock protein 90) complex, where it is then translocated to the RAS (protooncogene product p21) complex at the plasma
C Hodge et al.
The Journal of biological chemistry, 273(47), 31327-31336 (1998-11-13)
Growth hormone (GH), a major regulator of normal body growth and metabolism, regulates cellular gene expression. The transcription factors Elk-1 and Serum Response Factor are necessary for GH-stimulated transcription of c-fos through the Serum Response Element (SRE). GH stimulates the
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service