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Merck
CN

05-652

Anti-MUC1/episialin Antibody, clone 214D4

clone 214D4, Upstate®, from mouse

Synonym(s):

Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, Episialin, H23AG, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin, CD227

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
214D4, monoclonal
Application:
FACS, IHC
Citations:
17

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

clone

214D4, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable

isotype

IgG1

NCBI accession no.

NM_182741

UniProt accession no.

P15941

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

General description

none given

Immunogen

Fusion protein, human b-gal-episialin/MUC1 repeat domain

Application

Anti-MUC1/episialin Antibody, clone 214D4 detects level of MUC1/episialin & has been published & validated for use in FC, IH.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Biochem/physiol Actions

MUC1/episialin

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein G Chromatography

Preparation Note

2 years at -20°C

Analysis Note

routinely evaluated by immunohistochemistry

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

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Timothy D Blalock et al.
Investigative ophthalmology & visual science, 49(5), 1864-1871 (2008-04-26)
Three membrane-associated mucins (MAMs)--MUC1, MUC4, and MUC16--are expressed at the ocular surface epithelium. Soluble forms of MAMs are detected in human tears, but the mechanisms of their release from the apical cells are unknown. The purpose of this study was
A mechanism for inhibition of E-cadherin-mediated cell-cell adhesion by the membrane-associated mucin episialin/MUC1.
Wesseling, J, et al.
Molecular Biology of the Cell, 7, 565-577 (1996)
Ilene K Gipson et al.
PloS one, 9(6), e100393-e100393 (2014-06-27)
Membrane-anchored mucins are present in the apical surface glycocalyx of mucosal epithelial cells, each mucosal epithelium having at least two of the mucins. The mucins have been ascribed barrier functions, but direct comparisons of their functions within the same epithelium
Monoclonal antibodies against the nonmucin domain of MUC1/episialin.
Hilkens, J and Boer, M
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 19 Suppl 1, 67-70 (1998)
Pablo Argüeso et al.
The Journal of biological chemistry, 284(34), 23037-23045 (2009-06-27)
Maintenance of an intact mucosal barrier is critical to preventing damage to and infection of wet-surfaced epithelia. The mechanism of defense has been the subject of much investigation, and there is evidence now implicating O-glycosylated mucins on the epithelial cell
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