05-637-AF488
Anti-Bmi-1 Antibody, clone F6, Alexa Fluor™ 488
clone F6, from mouse, ALEXA FLUOR™ 488
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Synonym(s):
Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51, Bmi-1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
F6, monoclonal
species reactivity
human, monkey
species reactivity (predicted by homology)
rabbit (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BMI1(648)
General description
Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.
Immunogen
Epitope: a.a. 1-202
Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.
This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor™ 488 is validated for use in Immunocytochemistry for the detection of Bmi-1.
Quality
Evaluated by Immunocytochemistry in HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HepG2 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HepG2 cells.
Target description
40-44 kDa observed
Physical form
Protein G Purified
Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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