Anti-Bmi-1 Antibody, clone F6

The Bmi1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors.
Several lines of evidence implicate Bmi1 in tumorigenesis. Firstly, Bmi1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. Secondly, Bmi1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene leads to neoplastic transformation. Thirdly, the BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. Fourthly, the transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires Bmi1. Indeed, based on a list of genes differentially expressed in a mouse model of metastatic prostate cancer on a wild-type and bmi1-deficient genetic background a gene signature was recently identified that may predict response to therapy and survival in multiple types of cancer.

Recognizes Bmi-1 (triplet).

Immunized with recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Anti-Bmi-1 Antibody, clone F6 is a Mouse Monoclonal Antibody for detection of Bmi-1 also known as Polycomb group RING finger protein 4, RING finger protein 51 and has been validated in ICC, IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

routinely evaluated by immunoblot on RIPA lysates from U2OS cells.

40-44 kDa

Format: Purified

Immunoaffinity Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.

Protein G Purified

Stable for 1 year at 2-8°C from date of receipt.

Control
U2OS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos

feature_concentration_valuePlease refer to lot specific datasheet.

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