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Key Documents

Safety Information

05-594

Sigma-Aldrich

Anti-HSP90 Antibody

clone D7a, Upstate®, from mouse

Synonym(s):

Anti-EL52, Anti-HEL-S-65p, Anti-HSP86, Anti-HSP89A, Anti-HSP90A, Anti-HSP90N, Anti-HSPC1, Anti-HSPCA, Anti-HSPCAL1, Anti-HSPCAL4, Anti-HSPN, Anti-Hsp103, Anti-Hsp89, Anti-Hsp90, Anti-LAP-2, Anti-LAP2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

D7a, monoclonal

species reactivity

human, rabbit, pig, mouse, rat, bovine, chicken

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

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PHR1107G8415G1251
technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

microbiological culture: suitable

application(s)

cleaning products
cosmetics
flavors and fragrances
food and beverages
personal care

application(s)

cleaning products
cosmetics
food and beverages
personal care
pharmaceutical (small molecule)

application(s)

pharmaceutical (small molecule)

application(s)

-

format

neat

format

neat

format

-

format

-

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

200

grade

certified reference material, TraceCERT®

grade

certified reference material, pharmaceutical secondary standard

grade

-

grade

-

manufacturer/tradename

Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

Specificity

Recognizes HSP90.

Immunogen

Full-length protein purified from chicken brain; clone D7a

Application

This Anti-HSP90 Antibody is validated for use in IP, WB for the detection of HSP90.

Quality

routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts or human A431 cells

Target description

90 kDa

Physical form

Format: Purified
Protein G Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Information

常规特殊物品

  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Miquéias Lopes-Pacheco et al.
    Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6), 2194-2210 (2017-04-28)
    Premature degradation of mutated cystic fibrosis transmembrane conductance regulator (CFTR) protein causes cystic fibrosis (CF), the commonest Mendelian disease in Caucasians. Despite recent advances in precision medicines for CF patients, many CFTR mutants have not been characterized and the effects
    Interaction between the Rous sarcoma virus transforming protein and two cellular phosphoproteins: analysis of the turnover and distribution of this complex.
    Brugge, J, et al.
    Molecular and cellular biology, 3, 9-19 (1983)
    Qingming Kong et al.
    Proteome science, 8, 29-29 (2010-06-11)
    Infectious bronchitis virus (IBV) is the coronavirus of domestic chickens causing major economic losses to the poultry industry. Because of the complexity of the IBV life cycle and the small number of viral structural proteins, important virus-host relationships likely remain
    The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors.
    Pratt, W B
    Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 217, 420-434 (1998)
    Jason M Shapiro et al.
    PloS one, 11(11), e0165227-e0165227 (2016-11-02)
    We previously isolated AF20, a murine monoclonal antibody that recognizes a cell surface glycoprotein of approximately 90-110 kDa. The AF20 antigen is specifically expressed in human hepatoma and colon cancer cell lines, and thus could serve as a cancer biomarker.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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