05-531
Anti-His Tag Antibody
clone 4D11, mouse monoclonal, Upstate
Sign Into View Organizational & Contract Pricing
All Photos(1)
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Recommended Products
product name
Anti-Histidine Tagged Antibody, clone 4D11, clone 4D11, Upstate®, from mouse
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
4D11, monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HRG(3273)
Specificity
Recognizes tag sequence HHHHHHGS on either the N- or C-terminus of recombinant proteins; GS amino acids are required for recognition of 6His tag
Immunogen
N-terminal His-tagged and truncated TRAP protein corresponding to amino acids 26-248 from P. falciparum expressed in E coli.
Application
This Anti-Histidine Tagged Antibody (anti His antibody) clone 4D11 is validated for use in IC, WB for the detection of Histidine Tagged.
Quality
routinely evaluated by immunoblot on recombinant N-terminal and C-terminal His-tagged proteins
Target description
The apparent molecular weight is related to the tagged protein.
Physical form
Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.014M phosphate buffer, pH 7.6, 0.175M NaCl, and 0.07% sodium azide.
Analysis Note
Control
Cells expressing the His-tag
Cells expressing the His-tag
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ruiping Zheng et al.
Journal of cell science, 123(Pt 21), 3734-3744 (2010-10-14)
In higher eukaryotic cells, long non-protein-coding RNAs (lncRNAs) have been implicated in a wide array of cellular functions. Cell- or tissue-specific expression of lncRNA genes encoded in the mammalian genome is thought to contribute to the complex gene networks needed
Lisa A Mitchell et al.
Molecular human reproduction, 14(4), 235-243 (2008-02-05)
The process of capacitation is a pre-requisite for mammalian spermatozoa allowing them to gain the ability to fertilize an oocyte. A fundamental part of this mechanism is a dramatic increase in the level of tyrosine phosphorylation. Implicated in this process
K J Robson et al.
The EMBO journal, 14(16), 3883-3894 (1995-08-15)
Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much
Francesca Mancini et al.
The EMBO journal, 28(13), 1926-1939 (2009-06-13)
MDM4 is a key regulator of p53, whose biological activities depend on both transcriptional activity and transcription-independent mitochondrial functions. MDM4 binds to p53 and blocks its transcriptional activity; however, the main cytoplasmic localization of MDM4 might also imply a regulation
Wei-Hang Huang et al.
Heliyon, 9(11), e21486-e21486 (2023-11-29)
Originally extracted from Momordica charantia seeds, the antiviral and anti-tumor activities of Momordica anti-HIV protein MAP30 have become well known. Although MAP30 has been reported to possess antiviral activity against several human viruses, the current understanding of the MAP30-mediated antiviral
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service