05-516
Anti-Ras Antibody, clone RAS10
clone RAS10, Upstate®, from mouse
Sign Into View Organizational & Contract Pricing
All Photos(4)
Synonym(s):
GTP- and GDP-binding peptide B, GTPase Hras, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, Transforming protein p2, c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, p19 H-RasIDX protein, transformation gene: oncogene HAMSV,
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RAS10, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... HRAS(3265) , KRAS(3845) , NRAS(4893)
Related Categories
General description
The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.
Specificity
Other species unknown.
Immunogen
Purified recombinant Ras. Clone RAS10.
Application
Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.
Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.
Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.
Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.
1-4 μg of this antibody has been reported to immunoprecipitate Ras.
Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.
Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.
Quality
Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.
Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.
Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.
Target description
21 kDa
Linkage
Replaces: CBL751
Physical form
Format: Purified
Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
recommended
Product No.
Description
Pricing
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Oncogenic Ras in tumour progression and metastasis.
Giehl, Klaudia
Biological Chemistry, 386, 193-205 (2005)
The Ras-MAPK signal transduction pathway, cancer and chromatin remodeling.
Dunn, Katherine L, et al.
Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire, 83, 1-14 (2005)
Oncogenic Ras and its role in tumor cell invasion and metastasis.
Campbell, Paul M and Der, Channing J
Seminars in cancer biology, 14, 105-114 (2004)
Ras plasma membrane signalling platforms.
Hancock, John F and Parton, Robert G
The Biochemical Journal, 389, 1-11 (2005)
GTP hydrolysis mechanism of Ras-like GTPases.
Li, Guangpu and Zhang, Xuejun C
Journal of Molecular Biology, 340, 921-932 (2004)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service