05-511
Anti-TCF-4 Antibody, clone 6H5-3
clone 6H5-3, Upstate®, from mouse
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Synonym(s):
HMG box transcription factor 4, Transcription factor 7-like 2, T-Cell factor 4, T-cell-specific, HMG box, T-Cell-specific transcription factor 4, Transcription factor 7-like 2
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6H5-3, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
electrophoretic mobility shift assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
GenBank accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TCF7L2(6934)
General description
TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.
Specificity
This antibody recognizes TCF-4
Immunogen
Amino acids 31-331 of human TCF-4.
Application
Immunocytochemistry/Immunohistochemistry:
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).
Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001).
Supershift Assay:
A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).
Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001).
Supershift Assay:
A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).
Use Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2.
Quality
Routinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).
Western Blot Analysis:
0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).
Target description
Mr 66 kDa
Linkage
Replaces: 04-1080
Physical form
Format: Purified
Purified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
Analysis Note
Control
DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Disease-associated loci are significantly over-represented among genes bound by transcription factor 7-like 2 (TCF7L2) in vivo.
Zhao, J; Schug, J; Li, M; Kaestner, KH; Grant, SF
Diabetologia null
Differential activity of Wnt/beta-catenin signaling in the embryonic mouse thalamus.
Bluske, KK; Kawakami, Y; Koyano-Nakagawa, N; Nakagawa, Y
Developmental Dynamics null
Plakoglobin (gamma-catenin) has TCF/LEF family-dependent transcriptional activity in beta-catenin-deficient cell line.
Maeda, O; Usami, N; Kondo, M; Takahashi, M; Goto, H; Shimokata, K; Kusugami, K; Sekido, Y
Oncogene null
Control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines.
Beildeck, ME; Islam, M; Shah, S; Welsh, J; Byers, SW
Testing null
S P Rockman et al.
The Journal of biological chemistry, 276(48), 45113-45119 (2001-09-27)
Activation of beta-catenin/T cell factor (TCF) transcription as a result of mutations in the adenomatous polyposis coli (APC) and/or beta-catenin genes occurs in the majority of colon tumors. An increasing number of genes, including c-myc and cyclin D1, have been
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