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Merck
CN

05-511

Anti-TCF-4 Antibody, clone 6H5-3

clone 6H5-3, Upstate®, from mouse

Synonym(s):

HMG box transcription factor 4, Transcription factor 7-like 2, T-Cell factor 4, T-cell-specific, HMG box, T-Cell-specific transcription factor 4, Transcription factor 7-like 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
6H5-3, monoclonal
Application:
electrophoretic mobility shift assay
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
mouse, human
Citations:
122
Technique(s):
electrophoretic mobility shift assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
Q9NQB0

Key Documents

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Product Name

Anti-TCF-4 Antibody, clone 6H5-3, clone 6H5-3, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6H5-3, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

electrophoretic mobility shift assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

GenBank accession no.

CAB97213.1

UniProt accession no.

Q9NQB0

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TCF7L2(6934)

Analysis Note

Control
DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.
Routinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).

Application

Immunocytochemistry/Immunohistochemistry:
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).

Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001).

Supershift Assay:
A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).
Use Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2.

Biochem/physiol Actions

This antibody recognizes TCF-4

General description

Mr 66 kDa
TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

Immunogen

Amino acids 31-331 of human TCF-4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1080

Physical form

Format: Purified
Purified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Control of TCF-4 expression by VDR and vitamin D in the mouse mammary gland and colorectal cancer cell lines.
Beildeck, ME; Islam, M; Shah, S; Welsh, J; Byers, SW
Testing null
Plakoglobin (gamma-catenin) has TCF/LEF family-dependent transcriptional activity in beta-catenin-deficient cell line.
Maeda, O; Usami, N; Kondo, M; Takahashi, M; Goto, H; Shimokata, K; Kusugami, K; Sekido, Y
Oncogene null
Differential activity of Wnt/beta-catenin signaling in the embryonic mouse thalamus.
Bluske, KK; Kawakami, Y; Koyano-Nakagawa, N; Nakagawa, Y
Developmental Dynamics null
Disease-associated loci are significantly over-represented among genes bound by transcription factor 7-like 2 (TCF7L2) in vivo.
Zhao, J; Schug, J; Li, M; Kaestner, KH; Grant, SF
Diabetologia null
Meera Shah et al.
Cell cycle (Georgetown, Tex.), 14(3), 323-332 (2015-02-07)
Deregulated Wnt/β-catenin signaling promotes colorectal cancer (CRC) by activating expression of the c-MYC proto-oncogene (MYC). In the nucleus, the β-catenin transcriptional co-activator binds T-cell factor (TCF) transcription factors, and together TCF/β-catenin complexes activate MYC expression through Wnt responsive DNA regulatory
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