05-421-AF488
Anti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor™ 488 Conjugate
clone 1D6, from mouse, ALEXA FLUOR™ 488
Synonym(s):
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, PP2Ac, PP2Calpha, Replication protein C, RP-C
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
1D6, monoclonal
species reactivity
Xenopus, rabbit, human, bovine, rat, yeast, mouse
technique(s)
immunocytochemistry: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PPP2CA(5515)
General description
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (EC 3.1.3.16; UniProt P67775; PP2A-alpha, PP2Ac, PP2Calpha, Replication protein C, RP-C) is encoded by the PPP2CA (also known as PP2CA) gene (Gene ID 5515) in human. Protein phosphatase 2 (PP2 or PP2A) is a ubiquitously expressed heterotrimeric serine/threonine protein phosphatase with broad substrate specificity, such as Raf, MEK, and AKT. PP2A is composed of a structural A and a catalytic C subunit as the dimeric core enzyme, and a regulatory B subunit. There exist two different A subunits (PPP2R1A & PPP2R1B), two different C subunits (PPP2CA & PPP2CB), and 12 different B subunits (PPP2R2B-D, PPP2R3A-C, PPP2R4, PPP2R5A-E). The enzymatic activity of a catalytic subunit is altered upon binding an A subunit even in the absence of a B subunit. The B subunits are believed to play key roles in controlling the localization and specific activity of the A/C holoenzymes.
Specificity
Expected to recognize both spliced isoforms of human PP2A catalytic subunit reported by UniProt (P67775).
Immunogen
A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A).
Epitope: Amino acids 295-309.
Application
Anti-PP2A, C subunit Antibody, clone 1D6, Alexa Fluor™ 488 Conjugate is an antibody against PP2A for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PP2A in NIH/3T3 cells and HUVECs.
The unconjugated antibody (Cat. No. 05-421) is shown to be suitable also for immunocytochemistry, immunoprecipitation, and Western blotting applications.
The unconjugated antibody (Cat. No. 05-421) is shown to be suitable also for immunocytochemistry, immunoprecipitation, and Western blotting applications.
Research Category
Signaling
Signaling
Research Sub Category
Kinases & Phosphatases
Kinases & Phosphatases
Quality
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected PP2A in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected PP2A in A431 cells.
Target description
~36 kDa observed. 35.59 kDa (isoform 1) and 29.74 kDa (isoform 2) calculated.
Physical form
Protein A purified
Purified mouse monoclonal IgG2bκ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Yulia Gryaznova et al.
The EMBO journal, 40(7), e106797-e106797 (2021-03-02)
Partitioning of the genome in meiosis occurs through two highly specialized cell divisions, named meiosis I and meiosis II. Step-wise cohesin removal is required for chromosome segregation in meiosis I, and sister chromatid segregation in meiosis II. In meiosis I
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