05-182
Anti-FAK Antibody, clone 2A7
clone 2A7, Upstate®, from mouse
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2A7, monoclonal
species reactivity
human, mouse, rat, avian
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PTK2(5747)
Specificity
Recognizes FAK.
Immunogen
Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing p125FAK.
Application
Anti-FAK Antibody, clone 2A7 detects level of FAK & has been published & validated for use in IC & IP.
Not recommended in Western Blot
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Quality
routinely evaluated by immunoprecipitation of FAK from a mouse 3T3/A31 RIPA cell lysate, subsequently immunobloted with Anti-FAK (Catalog #06-543)
Target description
125 kDa
Linkage
Replaces: 04-591
Physical form
Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in Purified immunoglobulin in 10mM PBS, pH 7.4 containing no preservatives.
Storage and Stability
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Expressed in most tissue, 3T3/A31 cell lysate
Expressed in most tissue, 3T3/A31 cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 29(12), 4840-4852 (2015-08-08)
The phosphatase and tensin homolog deleted on chromosome 10 (PTEN) tumor suppressor protein is regulated by various mechanisms that are not fully understood. This includes regulation by Tyr phosphorylation by a mechanism that remains elusive. Here, we show that focal
The Journal of biological chemistry, 272(45), 28720-28725 (1997-11-14)
pp125 focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase transducing signals initiated by integrin engagement and G protein-coupled receptors, is highly expressed in brain. FAK from brain had a higher molecular weight and an increased autophosphorylation activity, than from other
Ambivalent role of pFAK-Y397 in serous ovarian cancer--a study of the OVCAD consortium.
Molecular Cancer null
Tyrosine phosphorylation of paxillin and focal adhesion kinase during insulin-like growth factor-I-stimulated lamellipodial advance.
The Journal of Biological Chemistry, 272, 5214-5218 (1997)
Frontiers in cellular and infection microbiology, 6, 175-175 (2016-12-21)
Type III secretion systems (T3SS) are dedicated to targeting anti-host effector proteins into the cytosol of the host cell to promote bacterial infection. Delivery of the effectors requires three specific translocator proteins, of which the hydrophilic translocator, LcrV, is located
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