05-175
Anti-Myb Antibody, clone 1-1
clone 1-1, Upstate®, from mouse
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All Photos(4)
Synonym(s):
Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA), v-myb avian myeloblastosis viral oncogene homolog, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral on
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1-1, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MYB(4602)
General description
Myb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5′-YAAC[GT]G-3′. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
Specificity
This antibody recognizes Myb, Mr 80 kDa, containing C-terminal amino acids from 401-636 (Salomoni, P., 1997).
Immunogen
Recombinant murine Myb purified by SDS-PAGE (Nicot, C., 2001) Clone 1-1.
Application
Anti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.
Immunoprecipitation:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.
Immunohistochemistry:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.
Immunohistochemistry:
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Routinely evaluated on RIPA lysate from Jurkat cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.
Target description
80 kDa
Physical form
Ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography.
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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cDNA array analysis of alterations in gene expression in the promyelocytic leukemia cell line, HL-60, after apoptosis induction with etoposide.
M Bj?rling-Poulsen, O-G Issinger
Apoptosis null
c-Maf interacts with c-Myb to down-regulate Bcl-2 expression and increase apoptosis in peripheral CD4 cells.
Siying Peng,Saif Lalani,Jianmei W Leavenworth,I-Cheng Ho,Mary E Pauza
European Journal of Immunology null
XinJing Tang et al.
Nucleic acids research, 36(2), 559-569 (2007-12-07)
Light-activated antisense oligodeoxynucleotides (asODNs) were developed to control the degradation of target mRNA in living cells by RNase H. A 20-mer asODN previously shown to target c-myb, a hematopoietic transcription factor, was covalently attached via a photocleavable linker (PL) to
Delivery of c-myb antisense oligodeoxynucleotides to human neuroblastoma cells via disialoganglioside GD(2)-targeted immunoliposomes: antitumor effects.
G Pagnan, D D Stuart, F Pastorino, L Raffaghello, P G Montaldo, T M Allen, B Calabretta, M Ponzoni
Journal of the National Cancer Institute null
Zhenglong Wu et al.
Journal of experimental & clinical cancer research : CR, 32, 98-98 (2013-12-03)
Mir-29 microRNA families are involved in regulation of various types of cancers. Although Mir-29 was shown to play an inhibitory role in tumorigenesis, the role of Mir-29 in breast cancer still remains obscure. In this study, we showed that Mir-29a
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