05-1592
Anti-mTOR Antibody, clone 21D8.2
clone 2ID8.2, from mouse
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All Photos(4)
Synonym(s):
FK506 binding protein 12-rapamycin associated protein 1, FK506 binding protein 12-rapamycin associated protein 2, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP-rapamycin associated protein, FKBP12-rapamycin complex-associated prot
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2ID8.2, monoclonal
species reactivity
mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MTOR(2475)
General description
mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
Specificity
This antibody recognizes mTOR.
Immunogen
Flag-tagged full-length mTOR protein
Application
Anti-mTOR Antibody, clone 21D8.2 is an antibody against mTOR for use in WB & IC.
Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a previous lot detected mTOR in C2C12, HeLa, and NIH/3T3 cell lysates.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Quality
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on HEK293 cell lysate.
Target description
289 kDa
Linkage
Replaces: 04-385
Physical form
Format: Purified
Protein G Purified
Purified immunoglobulin containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl and 0.05% Sodium Azide.
Storage and Stability
Maintain refrigerated at 2-8°C for 1 year from date of receipt.
Analysis Note
Control
Hek293 cell lysate
Hek293 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sidney Abou Sawan et al.
American journal of physiology. Cell physiology, 315(4), C537-C543 (2018-08-23)
We have recently demonstrated that whole egg ingestion induces a greater muscle protein synthetic (MPS) response when compared with isonitrogenous egg white ingestion after resistance exercise in young men. Our aim was to determine whether whole egg or egg white
Joseph J Bass et al.
Molecular metabolism, 42, 101059-101059 (2020-08-11)
The Vitamin D receptor (VDR) has been positively associated with skeletal muscle mass, function and regeneration. Mechanistic studies have focused on the loss of the receptor, with in vivo whole-body knockout models demonstrating reduced myofibre size and function and impaired muscle
Kuo-Hui Su et al.
Nature cell biology, 18(5), 527-539 (2016-04-05)
To cope with proteotoxic stress, cells attenuate protein synthesis. However, the precise mechanisms underlying this fundamental adaptation remain poorly defined. Here we report that mTORC1 acts as an immediate cellular sensor of proteotoxic stress. Surprisingly, the multifaceted stress-responsive kinase JNK
Somayeh Ahmadiantehrani et al.
Learning & memory (Cold Spring Harbor, N.Y.), 25(6), 273-282 (2018-05-17)
Nonassociative learning is considered simple because it depends on presentation of a single stimulus, but it likely reflects complex molecular signaling. To advance understanding of the molecular mechanisms of one form of nonassociative learning, habituation, for ethologically relevant signals we
Yong-Yan Bi et al.
Molecular medicine reports, 17(1), 1093-1098 (2017-11-09)
Accumulating data strongly suggests that leukocyte immunoglobulin like receptor B1 (PirB) inhibits axonal outgrowth. However, the underlying mechanisms remain unclear. In the present study, cortical neurons of newborn mice were cultured with Nogo‑66 (Nogo‑p4; 4 µmol/l; a PirB ligand) together with
Articles
Autophagy is a regulated process involved in cell growth, development, and recycling of cytoplasmic components in cells.
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