05-1570
Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3
clone 6D3, from rat
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Synonym(s):
PR domain containing 1, with ZNF domain, PR-domain zinc finger protein 1, beta-interferon gene positive-regulatory domain I binding factor, Positive regulatory domain I-binding factor 1, PRDI-binding factor-1, Beta-interferon gene positive regulatory dom
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6D3, monoclonal
species reactivity
mouse, human
technique(s)
ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PRDM1(639)
Related Categories
General description
B-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.
Specificity
Recognizes BLIMP-1
Immunogen
Epitope: Recognizes epitope located between amino acids 255-395 of BLIMP-1
Recombinant protein corresponding to mouse BLIMP-1.
Application
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Research Category
Epigenetics & Nuclear Function
Inflammation & Immunology
Epigenetics & Nuclear Function
Inflammation & Immunology
Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.
Quality
Evaluated by Western Blot in HL-60 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.
Target description
~ 95 kDa observed
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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