05-157
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9
clone 1B3B9, Upstate®, from mouse
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Synonym(s):
Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK 2, Mitogen-activated protein kinase 2, extracellular signal-regulated kinase-2, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2.
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1B3B9, monoclonal
species reactivity
avian, mouse, human, rat
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MAPKAPK2(9261)
mouse ... Mapkapk2(17164)
rat ... Mapkapk2(289014)
General description
ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.
Specificity
Other species cross reactivity is unknown.
This antibody recognizes MAP kinase encoded by the mapk gene, Mr 42 kDa.
Immunogen
HPLC purified murine recombinant MAP Kinase. Clone 1B3B9
Application
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 is an antibody against MAP Kinase 2/Erk2 for use in IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Quality
Routinely evaluated in RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts, or rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.
Target description
42 kDa
Linkage
Replaces: 04-349
Physical form
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide. Store frozen at -20°C, avoid freeze thaw cycles.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Cytosolic phospholipase A2 is phosphorylated in collagen- and thrombin-stimulated human platelets independent of protein kinase C and mitogen-activated protein kinase.
Borsch-Haubold, A G, et al.
The Journal of Biological Chemistry, 270, 25885-25892 (1995)
Reversion of Ras- and phosphatidylcholine-hydrolyzing phospholipase C-mediated transformation of NIH 3T3 cells by a dominant interfering mutant of protein kinase C lambda is accompanied by the loss of constitutive nuclear mitogen-activated protein kinase/extracellular signal-regulated kinase activity.
Bjorkoy, G, et al.
The Journal of Biological Chemistry, 272, 11557-11565 (1997)
Hitoshi Sase et al.
Journal of cell science, 122(Pt 18), 3303-3311 (2009-08-27)
Vascular endothelial growth factor receptor 2 (VEGFR2) plays crucial roles in vasculogenesis, a process involving cell proliferation, migration and differentiation. However, the molecular mechanism by which VEGFR2 signaling directs vascular endothelial differentiation of VEGFR2(+) mesodermal progenitors is not well understood.
Mitogen-activated protein kinase modulates ethanol inhibition of cell adhesion mediated by the L1 neural cell adhesion molecule.
Dou, X; Wilkemeyer, MF; Menkari, CE; Parnell, SE; Sulik, KK; Charness, ME
Proceedings of the National Academy of Sciences of the USA null
Kirsten Hattermann et al.
eLife, 5, e10820-e10820 (2016-01-23)
The transmembrane chemokines CX3CL1/fractalkine and CXCL16 are widely expressed in different types of tumors, often without an appropriate expression of their classical receptors. We observed that receptor-negative cancer cells could be stimulated by the soluble chemokines. Searching for alternative receptors
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