05-1321
Anti-BMI1 Antibody, clone AF27
clone AF27, from mouse
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Synonym(s):
B lymphoma Mo-MLV insertion region 1 homolog, B lymphoma Mo-MLV insertion region 1 homolog (mouse), BMI1 polycomb ring finger oncogene, Polycomb group RING finger protein 4, RING finger protein 51, murine leukemia viral (bmi-1) oncogene homolog, oncogene
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
AF27, monoclonal
species reactivity
human
technique(s)
ChIP: suitable
immunoprecipitation (IP): suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BMI1(648)
General description
The BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.
Specificity
This antibody recognizes BMI1.
Immunogen
MBP-tagged recombinant protein corres-ponding to full length human BMI1.
Application
Anti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP.
Chromatin Immunoprecipitation (ChIP):
A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).
A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Quality
Evaluated by Immunoprecipitation in MEF1 cell lysate.
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated BMI1 from 100 μg of MEF1 cell lysate.
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated BMI1 from 100 μg of MEF1 cell lysate.
Target description
~35 kDa
Physical form
Anti-BMI1, clone AF27
Format: Purified
Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable at 2-8°C for 1 year from date of receipt.
Analysis Note
Control
MEF1 cell lysate
MEF1 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
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Hsan-au Wu et al.
The Journal of biological chemistry, 288(51), 36398-36408 (2013-11-07)
Cbx7 is one of five mammalian orthologs of the Drosophila Polycomb. Cbx7 recognizes methylated lysine residues on the histone H3 tail and contributes to gene silencing in the context of the Polycomb repressive complex 1 (PRC1). However, our knowledge of
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