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Merck
CN

05-1313

Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4

clone HWA4C4, from mouse

Synonym(s):

ubiquitin C, polyubiquitin B, ubiquitin B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Key Documents

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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HWA4C4, monoclonal

species reactivity

vertebrates, human

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_018955.2

UniProt accession no.

P62988

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBAP2(55833)

General description

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
varies

Immunogen

Epitope: Lys63 ubiquitination site
Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).

Application

Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.
Research Category
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications

Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.

Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer′s patient.

ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.

Biochem/physiol Actions

Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.

Physical form

Format: Purified
Purified immunoglobulin by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Lys63 polyubiquitin chains.
Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein.
Galan, J M and Haguenauer-Tsapis, R
The Embo Journal, 16, 5847-5854 (1997)
Jun Meng et al.
Cell death & disease, 8(6), e2866-e2866 (2017-06-09)
Enterovirus 71 (EV71) is the main causative agent of hand, foot and mouth disease (HFMD), which induces significantly elevated levels of cytokines and chemokines, leading to local or system inflammation and severe complications, whereas the underlying regulatory mechanisms and the
Xingguang Liu et al.
Cell death and differentiation, 24(4), 683-693 (2017-02-06)
Lung ischemia-reperfusion (I/R) injury remains one of the most common complications after various cardiopulmonary surgeries. The inflammation response triggered by the released damage-associated molecular patterns (DAMPs) aggravates lung tissue damage. However, little is known about the role of autophagy in
Lys63-linked polyubiquitin chains: linking more than just ubiquitin.
Kawadler, Holli and Yang, Xiaolu
Cancer Biology & Therapy, 5, 1273-1274 (2006)
I-Ting Chen et al.
Scientific reports, 5, 12300-12300 (2015-07-21)
Toll-like receptor 4 (TLR4) plays an important role in innate immunity by eliciting inflammation. Upon receptor engagement, transforming growth factor β-activated kinase 1 (TAK1) is an essential mediator that transmits a signal from the receptor to downstream effectors, IκB kinase
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