05-1098
Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2
clone 7D2.2, from mouse
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acetyl-CoA carboxylase 1, acetyl-CoA carboxylase-alpha, acetyl-Coenzyme A carboxylase alpha
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7D2.2, monoclonal
species reactivity
human, rat
technique(s)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ACACA(31)
rat ... Acaca(60581)
General description
Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activity of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormone glucagon or epinephrine binds to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.
Immunogen
Histidine-tagged recombinant protein corresponding to human acetyl CoA Carboxylase.
Application
Anti-acetyl CoA Carboxylase Antibody, clone 7D2.2 detects level of acetyl CoA Carboxylase & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Western Blot Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of rat heart lysate.
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Quality
Evaluated by Western Blot in human heart lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected acetyl CoA Carboxylase on 10 µg of human heart lysate.
Target description
~ 266 kDa Observed
Linkage
Replaces: 04-322
Physical form
Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human heart lysate
Human heart lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
recommended
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
American journal of physiology. Endocrinology and metabolism, 313(1), E48-E62 (2017-03-23)
AMP-activated protein kinase (AMPK) plays a key role in energy homeostasis and is activated in response to contraction-induced ATP depletion in skeletal muscle via a rise in intracellular AMP/ADP concentrations. AMP can be deaminated by AMP-deaminase (AMPD) to IMP, which
Frontiers in endocrinology, 12, 697445-697445 (2022-01-04)
We evaluated the efficacy of a novel brain permeable "metformin-like" AMP-activated protein kinase activator, R481, in regulating glucose homeostasis. We used glucose sensing hypothalamic GT1-7 neuronal cells and pancreatic αTC1.9 α-cells to examine the effect of R481 on AMPK pathway
iScience, 26(11), 108196-108196 (2023-11-09)
The liver coordinates the systemic response to nutrient deprivation and availability by producing glucose from gluconeogenesis during fasting and synthesizing lipids via de novo lipogenesis (DNL) when carbohydrates are abundant. Mitochondrial pyruvate metabolism is thought to play important roles in
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(3), 1163-1179 (2018-09-10)
Non-alcoholic fatty liver disease (NAFLD) encompasses a series of pathologic changes ranging from steatosis to steatohepatitis, which may progress to cirrhosis and hepatocellular carcinoma. The purpose of this study was to determine whether ganoderma lucidum polysaccharide peptide (GLPP) has therapeutic
Oncotarget, 7(24), 37103-37120 (2016-05-22)
The glycerophospholipid phosphatidylcholine is the most abundant phospholipid species of eukaryotic membranes and essential for structural integrity and signaling function of cell membranes required for cancer cell growth. Inhibition of choline kinase alpha (CHKA), the first committed step to phosphatidylcholine
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