04-953
Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal
culture supernatant, clone A5S, Upstate®
Synonym(s):
Anti-Anti-CHTD8, Anti-Anti-JV18, Anti-Anti-JV18-1, Anti-Anti-LDS6, Anti-Anti-MADH2, Anti-Anti-MADR2, Anti-Anti-hMAD-2, Anti-Anti-hSMAD2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
A5S, monoclonal
species reactivity
human, zebrafish, mouse, canine
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer465/pSer467)
Gene Information
human ... SMAD2(4087)
General description
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFb superfamily of modulators. Like many other TGFb family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFb′s. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Specificity
Predicted to cross-react with X. laevis based on sequence homology.
Recognizes phosphorylated Smad2. No Cross-reactivity with Smad3.
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids [pS]M[pS] surrounding sites Ser465/467 of human Smad2
Application
Detect phospho-Smad2 (Ser465/467) with Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S (Rabbit Monoclonal Antibody), that has been shown to work in WB.
Quality
Routinely evaluated by immunoblot analysis.
Target description
55-60 kDa
Linkage
Replaces: 05-953
Analysis Note
Control
HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ
HL60 cell lysate, HepG2 cells treated with TGFa, or HepG2 cells treated with TGFβ
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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