Anti-TBK1 Antibody, clone AOW9, rabbit monoclonal

TBK, also known as IκB kinase (IKK)-related kinase NAK, belong to the serine/threonine IKK kinase family and is thought to play a role in NF-kappa-B (NF-κB) activation. The NF-κB complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NF-κB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NF-κB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NF-κB inhibition caused by TANK.

Predicted to cross-react with mouse, dog, and chicken based on sequence homology.

TBK1; Does not cross-react with IKKε, IKKα or IKKβ.

Epitope: a.a. 642-645

Peptide corresponding to the sequence C...EEVS... (amino acids 642-645) of human TANK-binding kinase 1 (TBK1) with an N-terminal cysteine added for conjugation purposes.

Detect TBK1 using this Anti-TBK1 Antibody, clone AOW9 validated for use in WB & IP.

Immunoprecipitation:
1-4 μL of a previous lot immunoprecipitated TBK1 from 100 μg of HEK293 RIPA lysate.

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Kinases & Phosphatases

Immunological Signaling

Evaluated by western blot on RIPA lysates from HEK293 cells.

Western Blot Analysis:
A 1:500 to 1:2,000 of this antibody detected TBK1 in RIPA lysates from HEK293 cells.

85 kDa

Replaces: 05-856

Cultured supernatant containing 0.05% sodium azide.

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
RIPA lysates from HEK293 cells.

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