Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.



The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

17 kDa

Peptide containing the sequence [RT_trimKQ] in which lysine 4 is trimethylated on human histone H3

Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315 is a rabbit monoclonal antibody for detection of trimethyl-Histone H3 (Lys4) also known as H3K4me3, Histone H3 (tri methyl K4) & has been validated in WB, ChIP, DB, Mplex, ChIP-seq.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in human colon and human breast cancer tissue sections

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in HeLa, A431, HUVEC, and NIH3T3 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Histone H3 containing trimethyl-lysine 4 and, to a lesser extent, dimethyl-lysine 4. See COA for specificity data.

Cultured supernantant in 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
HeLa acid extract

routinely evaluated by immunoblot on in acid extracted proteins from HeLa cells, but not recombinant unmethylated Histone H3 (Catalog #14-494)

Replaces: 04-791

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