04-742
Anti-SHP-1/2 Antibody, clone NL213, rabbit monoclonal
culture supernatant, clone NL213, Upstate®
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Anti-DRPLA
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biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
NL213, monoclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PTPN6(5777)
mouse ... Ptpn6(15170)
Specificity
Others not tested.
Recognizes SHP-1 greater than SHP-2, Mr ~65 kDa
Immunogen
Full-length glutathione transferase SHP-1 fusion protein, purified from bacteria
Application
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
This Anti-SHP-1/2 Antibody, clone NL213 is validated for use in IP, WB for the detection of SHP-1/2.
Quality
routinely evaluated by immunoblot in RIPA lysates from human A431 carcinoma cells
Target description
~65kDa
Linkage
Replaces: 05-742
Physical form
Cultured supernantant in 0.05% sodium azide
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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American journal of physiology. Endocrinology and metabolism, 304(11), E1188-E1198 (2013-03-28)
Renal podocyte apoptosis is an early event of diabetic nephropathy progression. Insulin action is critical for podocyte survival. Previous studies demonstrated that Src homology-2 domain-containing phosphatase-1 (SHP-1) is elevated in renal cortex of type 1 diabetic mice; we hypothesized that
PloS one, 13(11), e0206364-e0206364 (2018-11-06)
Enhancement of hematopoietic stem cells (HSCs) proliferation is a central aim in bone marrow transplantation (BMT). A stem cell factor (SCF) and c-Kit mediated extracellular signaling trigger proliferation of HSCs. This signaling is negatively regulated by protein tyrosine phosphatases (PTPs)
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