04-1664
Anti-Histidine Tag Antibody, clone RM146
clone RM146, from rabbit
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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
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biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
RM146, monoclonal
species reactivity (predicted by homology)
all
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HRG(3273)
Specificity
This antibody reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.
Immunogen
Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.
Application
Immunoprecipitation Analysis (IP): 0.5-2 μg/mL from a representative lot detected Histidine Tag in HEK293T whole lysate control and IP by clone RM146.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
This Anti-Histidine Tag Antibody, clone RM146 is validated for use in Western Blotting, Immunoprecipitation for the detection of Histidine Tag.
Quality
Evaluated by Western Blotting in HEK293T cell lysate untransfected with His-Tag fusion protein X, transfected with His-Tag fusion protein X, E. coli lysate without His-Tag Protein Y, and E. coli lysate with His-Tag Protein Y.
Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.
Western Blotting Analysis (WB): 0.2 μg/mL of this antibody detected Histidine Tag in HEK293T cell lysate transfected with His-Tag fusion protein X and E. coli lysate with His-Tag Protein Y.
Target description
~52 and ~85 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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