04-1585
Anti-IL-18 Antibody, clone 12E7.1
clone 12E7.1, from mouse
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Synonym(s):
interleukin 18 (interferon-gamma-inducing factor), IL-1 gamma, IFN-gamma-inducing factor, iboctadekin, interleukin-18, Interleukin-1 gamma, Iboctadekin, Interferon gamma-inducing factor
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
12E7.1, monoclonal
species reactivity
human, mouse
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
isotype
IgMκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IL18(3606)
mouse ... Il18(16173)
Related Categories
General description
Interleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor α (IL-18α) to form a low affinity binary complex that recruits IL-18β to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon-γ synthesis from T-helper 1 and natural killer cells.
Immunogen
KLH-conjugated linear peptide corresponding to human IL-18.
Application
Anti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.
Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in human tonsil tissue.
Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.
Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
Cytokines & Cytokine Receptors
Quality
Evaluated by Flow Cytometry in mouse splenocytes.
Flow Cytometry Analysis: 1 g of this antibody detected IL-18 in mouse splenocytes.
Flow Cytometry Analysis: 1 g of this antibody detected IL-18 in mouse splenocytes.
Target description
~24 kDa observed
Physical form
Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse splenocytes
Mouse splenocytes
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Solène Gatault et al.
Journal of immunology (Baltimore, Md. : 1950), 195(5), 2483-2492 (2015-07-29)
Eosinophils are multifunctional leukocytes that are involved in innate and adaptive immune responses through the expression of various receptors and mediators. Previously, we showed that human eosinophils and T cells shared cytotoxic activities against tumor cells that involved the γ-δ
Zhaoxia Liang et al.
Journal of neuroinflammation, 16(1), 226-226 (2019-11-18)
Chemerin is highly expressed in the serum, placenta tissue, and umbilical cord blood of diabetic mother; however, the impact of chemerin on cognitive disorders of offspring from mothers with diabetes in pregnancy remains unclear. A diabetic phenotype in pregnant mice
Hung-Yu Chiang et al.
Nature communications, 13(1), 874-874 (2022-02-17)
IL-18 is emerging as an IL-22-induced and epithelium-derived cytokine which contributes to host defence against intestinal infection and inflammation. In contrast to its known role in Goblet cells, regulation of barrier function at the molecular level by IL-18 is much
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