04-1556
Anti-MDM4 Antibody, clone 7A8
clone 7A8, from mouse
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Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse), Mdm4, transformed 3T3 cell double minute 4, p53 binding
protein, Mdm4, transformed 3T3 cell double minute 4, p53 binding pr
protein, Mdm4, transformed 3T3 cell double minute 4, p53 binding pr
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7A8, monoclonal
species reactivity
mouse, human
technique(s)
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MDM4(4194)
mouse ... Mdm4(17248)
General description
MDM4 (sometimes referred to as MDMX) is a member of the MDM family proteins. It is known to inhibit p53 and p73 mediated cell cycle arrest and apoptosis. MDM4 is amplified or overexpressed in 10-20% of tumors and 65% of retinoblastomas. The full-length protein has significant homology to MDM2 and can also bind to and inhibit p53. In both MDM2 and MDM4, this interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Other similar domains include the C-terminal RING finger motif. Although these proteins share similar domains, MDM2 and MDM4 are non-redundant p53 inhibitors, since normal levels of either protein does not compensate for the loss of the other. Recent evidence suggests complementary activities for these proteins with MDM4 regulating p53 activity, and MDM2 playing a key role in p53 stability.
Specificity
This antibody recognizes MDM4.
Immunogen
Epitope: Unknown
His-tagged recombinant protein corresponding to mouse MDM4.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Transcription Factors
Apoptosis - Additional
Transcription Factors
Apoptosis - Additional
Use Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse).
Quality
Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM4 in 10 µg of HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM4 in 10 µg of HeLa cell lysate.
Target description
~ 80/27 kDa Observed. There are multiple forms for MDM4. Up to 7 different variants have been reported. Full legth of MDM4 is ~80kDa The short form of MDM4 (MDM4-S) migrates at approximately 17 and 27kDa (R. Rallapalli, G. Strachan, B. Cho, W. Mercer‡, and DJ. Hall, 1999, A Novel MDMX Transcript, THE JOURNAL OF BIOLOGICAL CHEMISTRY, 274(12), 8299–8308)
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HeLa cell lysate
HeLa cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Oncogenesis, 11(1), 37-37 (2022-07-03)
p53 is the most highly mutated tumor suppressor across multiple types of human cancers. The level and function of p53 are fine-tuned through multifaced mechanisms in which the protein-protein interaction between p53 and MDM2 is considered as a major circuit.
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