04-1501
Anti-B7-H3 (CD276) Antibody, clone MJ8
clone MJ8, from rat
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B7 homolog 3, CD276 antigen, CD276 molecule, Costimulatory molecule
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biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
MJ8, monoclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CD276(80381)
Related Categories
General description
B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.
Specificity
This antibody recognizes the extracellular domain of B7-H3 (CD276).
Immunogen
Epitope: Extracellular Domain
Mouse B7-H3 Ig2a FC fusion protein, consisting of the extracellular domain linked to the Fc portion of mouse IgG2a.
Application
Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Osamu, N., et al., 2008).
Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).
Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Quality
Evaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.
Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.
Target description
~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Human placenta tissue lysate
Human placenta tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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