04-148
Anti-CLEC9A Antibody, clone 10B4
clone 10B4, from rat
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C-type lectin domain family 9 member A, Dendritic cell natural killer lectin group receptor 1, CLEC9A, CLEC
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biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10B4, monoclonal
species reactivity
mouse
technique(s)
flow cytometry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Clec9A(232414)
Related Categories
General description
CLEC9A, also known as Dendritic cell natural killer lectin group receptor 1 (DNGR-1), and encoded by the gene Clec9a/Dngr-1, functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. CLEC9A functions as a SYK-coupled C-type lectin receptor to mediate sensing of necrosis by the principal dendritic-cell subset involved in regulating cross-priming to cell-associated antigens. CLEC9A recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediates the cross-presentation of dead-cell associated antigens in a Syk-dependent manner. CLEC9A is localized to the plasma membrane. CLEC9A is expressed in dendritic cells and has multiple isoforms; isoform 4 is expressed at high levels by CD8+ dendritic cells and at low levels by plasmacytoid DCs but not by other hematopoietic cells.
Immunogen
KLH-conjugated linear peptide corresponding to mouse CLEC9A.
Application
Detect CLEC9A using this rat monoclonal antibody, Anti-CLEC9A Antibody, clone 10B4 validated for use in western blotting & Flow Cytometry.
Flow Cytometry Analysis: A representative lot from an independent laboratory detected CLEC9A in mouse Conventional Dendritic cells and on most Plasmacytoid Dendritic cells (Caminschi, I., et al. (2008). Blood. 112(8):3264-3273.).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Quality
Evaluated by Western Blotting in mouse lymph node tissue lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected CLEC9A in 10 μg of mouse lymph node tissue lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected CLEC9A in 10 μg of mouse lymph node tissue lysate.
Target description
~27 kDa and ~16 kDa observed. Uniprot describes five isoforms at 27 kDa, 27 kDa, 30 kDa, 20 kDa, and 17 kDa
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Blood, 112(8), 3264-3273 (2008-08-02)
A novel dendritic cell (DC)-restricted molecule, Clec9A, was identified by gene expression profiling of mouse DC subtypes. Based on sequence similarity, a human ortholog was identified. Clec9A encodes a type II membrane protein with a single extracellular C-type lectin domain.
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