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04-079

Sigma-Aldrich

Anti-trimethyl-Histone H4 (Lys20), Antibody, rabbit monoclonal

culture supernatant, Upstate®

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Synonym(s):
H4K20me3, Histone H4 (tri methyl K20)
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

monoclonal

species reactivity

vertebrates, human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
dot blot: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_003538.3

UniProt accession no.

P62805

shipped in

wet ice

target post-translational modification

trimethylation (Lys20)

Gene Information

human ... H4C1(8359)

General description

Histones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.

Specificity

Recognizes Histone H4 when trimethylated on Lys20.

Immunogen

Peptide corresponding to human Histone H4 containing the sequence [HRmethKVL] on which lysine 20 is trimethylated.

Application

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 10 µL of either a negative control supernatant, or Anti-Trimethyl-Histone H4 (Lys20) antibody and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of trimethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using ChIP Primers B-Globin. Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.

Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-079, Anti-trimethyl Histone H4 (Lys20) (1:500 dilution). Proteins were visualized using a Donkey anti-rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Detect trimethyl-Histone H4 (Lys20) using this Anti-trimethyl-Histone H4 (Lys20) Antibody, rabbit demontrated performance in WB, ChIP & DB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by immunoblot.

Target description

11kDa

Physical form

100 μL of rabbit monoclonal IgG cell culture supernatant in 0.1% sodium azide

Storage and Stability

2 years at -20°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Volodymyr P Tryndyak et al.
Cancer biology & therapy, 5(1), 65-70 (2005-12-03)
Cancer cells are characterized by epigenetic dysregulation, including global genome hypomethylation, regional hypo- and hypermethylation, altered histone modifications, and disturbed genomic imprinting. Despite the long-established fact that global DNA hypomethylation is a common feature of tumors, very little is known
Dmitry Karachentsev et al.
Developmental biology, 304(1), 46-52 (2007-01-19)
Methylation of specific amino acids in histone tails is responsible for packaging DNA into condensed, repressed chromatin, and into open chromatin that is accessible to the transcription machinery. Monomethylation and trimethylation of histone H4-lysine 20 (H4-K20) control the formation of
David M Nelson et al.
Genome biology, 17(1), 158-158 (2016-07-28)
Histone modification H4K20me3 and its methyltransferase SUV420H2 have been implicated in suppression of tumorigenesis. The underlying mechanism is unclear, although H4K20me3 abundance increases during cellular senescence, a stable proliferation arrest and tumor suppressor process, triggered by diverse molecular cues, including
Histone trimethylation and the maintenance of transcriptional ON and OFF states by trxG and PcG proteins.
Papp, B; Muller, J
Genes & Development null

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