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03-0188-00

Millipore

SMC® Human Phospho-α-Synuclein (S129) High Sensitivity Immunoassay Kit

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Synonym(s):
p-α-synuclein S129 immunoassay kit, p-α-synuclein Ser129 immunoassay kit, p-α-synuclein Single Molecule Counting Kit, p-α-synuclein ultra-sensitive immunoassay
UNSPSC Code:
12161503
NACRES:
NA.84

species reactivity

mouse, monkey, cat, rat

manufacturer/tradename

SMC®

assay range

sensitivity: 0.1 pg/mL
(Lower Limit of Quantification)
inter-assay cv: 7%
intra-assay cv: 6%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)

technique(s)

single molecule counting: suitable

storage temp.

2-8°C

Related Categories

General description

The SMC® Human Phospho-a-Synuclein (S129) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Specificity

Species Reactivity:
Specificity to the following species analytes were tested with the following results:
Monkey – 100%
Cat – 100%
Rat – 100%
Mouse – 25%
Beagle – 25%

Cross Reactivity:
No cross-reactivity was observed to an alpha-synuclein recombinant protein purified from E. Coli (i.e. non-phosphorylated alpha-synuclein).

Accuracy:
CSF Recovery: 5 CSF samples spiked with average recovery of 100% and a range of 95-103%
Serum/Plasma Recovery: 2 serum samples and 3 plasma samples with average recovery of 87% and a range of 83-91%

Application

The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human phospho-a-synuclein (S129) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of phospho-a-synuclein (S129) in human CSF, serum, and plasma.

This new streamline kit has the following features including:
  • Improved options for sample prep and incubation
  • Lyophilization of the standard provides the kit at one storage condition
  • Removal of a plate transfer step
  • Increased reagent volumes to accommodate automation
  • Validated to run on both SMCxPRO® and Erenna


Research Category:
Neuroscience, neurological

Research Sub-Category:
  • Neurodegenerative Diseases
  • Neuronal & Glial Markers
  • Neuroscience
  • Nervous System


Packaging

96 well assay

Components

  • Assay Buffer
  • Phospho-a-synuclein (S129) Coated Beads
  • Standard Diluent
  • Phospho-a-synuclein (S129) Detection Antibody
  • Phospho-a-synuclein (S129) Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

Legal Information

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

WGK

WGK 3

Regulatory Information

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