03-0171-00
SMC® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit
Synonym(s):
Abrineurin immunoassay kit, BDNF Single Molecule Counting Kit, BDNF ultra-sensitive immunoassay
About This Item
manufacturer/tradename
SMC®
assay range
sensitivity: 0.04 pg/mL
(lower limit of quantification)
inter-assay cv: <11%
intra-assay cv: <10%
((Intra-assay CV n=20; Inter-assay n=5))
technique(s)
ELISA: suitable
single molecule counting: suitable
relevant disease(s)
Alzheimer′s disease
storage temp.
2-8°C
Related Categories
General description
The SMC® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.
Specificity
Cross reactivity and interference of ProBDNF was tested in this assay.
The assay shows 1.2% cross reactivity with ProBDNF. Serum and plasma spiked with ProBDNF show an average 4.4% interference.
Accuracy:
- Minimum of 5 EDTA plasma samples spiked with average recovery of 96% with a range of 81-108%
- Minimum of 5 serum samples spiked with average recovery of 90% with a range of 71-98%
- Minimum of 5 cerebrospinal fluid (CSF) samples spiked with average recovery of 98% with a range of 83-108%
Application
- Neuroscience
Research Sub-Category:
- Alzheimer′s Disease
- Neurochemistry
- Neurotrophins
- Signaling Neuroscience
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of BDNF in human EDTA plasma, serum, and cerebrospinal fluid.
Features and Benefits
- Improved options for sample prep and incubation
- Lyophilization of the standard provides the kit at one storage condition
- Removal of a plate transfer step
- Increased reagent volumes to accommodate automation
- Validated to run on both SMCxPRO® and Erenna
Components
- Assay Buffer
- BDNF Coated Beads
- Standard Diluent
- BDNF Detection Antibody
- BDNF Standard
- 10X Wash Buffer
- Buffer D
- Elution Buffer B
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation
Target Organs
Respiratory Tract
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Neuroscience biomarker research expands with high-sensitivity immunoassays for low-abundant biomarkers.
Explore high-sensitivity SMC® neurology immunoassays that precisely measure low levels of neurology protein analytes, such as NF-L or Tau proteins.
Explore ultrasensitive immunoassay technology with SMC® technology, including its biomarker detection platform, advantages of high sensitivity assays, and more.
Find immunoassays to explore neuroimmunology and neuroinflammation cytokines and neurodegenerative disease biomarkers.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service