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clone MB1.2, Chemicon®, from rat
clone mAb13, from rat
clone 12G10, Chemicon®, from mouse
clone 8E3, from mouse
Description
Synonyms
CD29 antigen, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, fibronectin receptor beta subunit, integrin VLA-4 beta subunit, integrin beta 1, integrin beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29
Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide
Integrin beta-1, Fibronectin receptor subunit beta, VLA-4 subunit beta, CD29
Quality Designations
Properties
NCBI accession no.:
NP_002202NCBI accession no.:
NP_596867NCBI accession no.:
NM_002211NCBI accession no.:
NP_002202UniProt accession no.:
P05556UniProt accession no.:
P05556UniProt accession no.:
P05556UniProt accession no.:
P05556antibody form:
purified immunoglobulinantibody form:
purified immunoglobulinantibody form:
purified immunoglobulinantibody form:
purified immunoglobulinbiological source:
ratbiological source:
ratbiological source:
mousebiological source:
mouseclone:
MB1.2, monoclonalclone:
mAb13, monoclonalclone:
12G10, monoclonalclone:
8E3, monoclonalGene Information:
human ... ITGB1(3688) Gene Information:
human ... ITGB1(3688) Gene Information:
human ... ITGB1(3688) Gene Information:
human ... ITGB1(3688) shipped in:
ambientshipped in:
ambientshipped in:
dry iceshipped in:
wet iceisotype:
IgG2κisotype:
IgG2aκisotype:
IgG1κisotype:
IgG1κmanufacturer/tradename:
Chemicon®manufacturer/tradename:
manufacturer/tradename:
Chemicon®manufacturer/tradename:
packaging:
antibody small pack of 25 μgpackaging:
antibody small pack of 25 μLpackaging:
packaging:
target post-translational modification:
unmodifiedtarget post-translational modification:
unmodifiedtarget post-translational modification:
unmodifiedtarget post-translational modification:
unmodifiedantibody product type:
primary antibodiesantibody product type:
primary antibodiesantibody product type:
primary antibodiesantibody product type:
primary antibodiesspecies reactivity (predicted by homology):
ratspecies reactivity (predicted by homology):
species reactivity (predicted by homology):
species reactivity (predicted by homology):
species reactivity:
mouse, humanspecies reactivity:
humanspecies reactivity:
humanspecies reactivity:
humanstorage temp.:
2-8°Cstorage temp.:
storage temp.:
storage temp.:
technique(s):
flow cytometry: suitableimmunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
technique(s):
immunohistochemistry: suitableimmunoprecipitation (IP): suitable
inhibition assay: suitable
technique(s):
ELISA: suitablecell culture | mammalian: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
technique(s):
activity assay: suitableimmunoprecipitation (IP): suitable
western blot: suitable
Quality Level:
100Quality Level:
100Quality Level:
100Quality Level:
100Usage and Safety
Application
Anti-Integrin β1 Antibody, clone MB1.2 is an antibody against Integrin β1 for use in FC, IH, IP & WB.Immunoprecipitation:
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
Anti-Integrin beta1 (CD29), clone mAb13, Cat. No. MABT821, is a rat monoclonal antibody that detects integrin beta-1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Inhibition studies.Inhibition Analysis: A representative lot blocked cell attachment in Inhibition studies (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).
Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).
Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
Inhibition Analysis: A representative lot blocked HT-1080 cells adhesion to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).
Research Category
Cell Structure
Immunocytochemistry Analysis: A representative lot detected Integrin beta1 (CD29) in Immunocytochemistry applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75; Humphries, J.D., et. al. (2005). J Biol Chem. 280(11):10234-43).
Immunoprecipitation Analysis: A representative lot detected Integrin beta1 (CD29) in Immunoprecipitation applications (Akiyama, S.K., et. al. (1989). J Cell Biol. 109(2):863-75).
Inhibition Analysis: A representative lot blocked HT-1080 cells adhesion to fibronectin coated plates (Courtesy of Dr. Kenneth M. Yamada, M.D., Ph.D., NIDCR, National Institutes of Health, Bethesda, MD USA).
Research Category
Cell Structure
Anti-Integrin β1 Antibody, clone 12G10 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IP, FUNC, CULT & WB.Flow cytometry: 1:25-1:50
ELISA : 10 μg/mL
Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.
Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.
Optimal working dilutions must be determined by end user.Research Category
Cell StructureResearch Sub Category
Integrins
ELISA : 10 μg/mL
Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.
Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.
Optimal working dilutions must be determined by end user.Research Category
Cell StructureResearch Sub Category
Integrins
Research Category
Cell StructureResearch Sub Category
ECM ProteinsThis Anti-Integrin beta1 Antibody is validated for use in WB, IP & activity assays for the detection of Integrin beta1.Western Blot Analysis: A 1:100,000 dilution from a representative lot detected Integrin protein in WB under non-reducing conditions.
Activity Assay Analysis: A representative lot from an independent laboratory stimulated cellular adhesion and increased ligand binding activity of Integrin Beta1 (Mould, A. P., et al. (2004). J Biol Chem. 280(56):4238-4226.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Integrin Beta1 in IP (Tiwari, S., et al. (2011). J Cell Sci. 124(10):1672-1680.).
Cell StructureResearch Sub Category
ECM ProteinsThis Anti-Integrin beta1 Antibody is validated for use in WB, IP & activity assays for the detection of Integrin beta1.Western Blot Analysis: A 1:100,000 dilution from a representative lot detected Integrin protein in WB under non-reducing conditions.
Activity Assay Analysis: A representative lot from an independent laboratory stimulated cellular adhesion and increased ligand binding activity of Integrin Beta1 (Mould, A. P., et al. (2004). J Biol Chem. 280(56):4238-4226.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Integrin Beta1 in IP (Tiwari, S., et al. (2011). J Cell Sci. 124(10):1672-1680.).
Risk and Safety
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 2
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 2
Flash Point(F):
does not flash
Flash Point(C):
does not flash
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 2
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
Storage Class Code:
12 - Non Combustible Liquids
WGK:
WGK 2
Flash Point(F):
Not applicable
Flash Point(C):
Not applicable
