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850500P

Avanti

CHAPS

Avanti Research™ - A Croda Brand

Synonym(s):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

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About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S
CAS Number:
75621-03-3
Molecular Weight:
614.88
UNSPSC Code:
12161902
NACRES:
NA.25

Assay

>99% (TLC)

form

powder

mol wt

614.88 g/mol

packaging

pkg of 1 × 1 g (850500P-1g)

manufacturer/tradename

Avanti Research™ - A Croda Brand

technique(s)

protein quantification: suitable

shipped in

dry ice

storage temp.

−20°C

SMILES string

C[C@@]12C(CC[C@@H]2[C@@H](CCC(NCCC[N+](C)(CCCS(=O)([O-])=O)C)=O)C)C3[C@H](O)C[C@]4([H])C[C@H](O)CC[C@]4(C)C3C[C@@H]1O

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-BCTGSCMUSA-N

General description

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Application

CHAPS is suitable to prepare bicelles and to study the processes of liposome solubilization and reconstitution of transport proteins.

Packaging

20 mL Clear Glass Screw Cap Vial (850500P-1g)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Functional reconstitution of the nicotinic acetylcholine receptor by CHAPS dialysis depends on the concentrations of salt, lipid, and protein
Schuerholz T, et al.
Biochemistry, 31(21), 5067-5077 (1992)
Bilayer membrane modulation of membrane type 1 matrix metalloproteinase (MT1-MMP) structure and proteolytic activity
Cerofolini L, et al.
Scientific reports, 6, 29511-29511 (2016)
Liposome solubilization and membrane protein reconstitution using Chaps and Chapso
Cladera J, et al.
European Journal of Biochemistry, 243(3), 798-804 (1997)
Stuart J Cordwell
Methods in molecular biology (Clifton, N.J.), 424, 139-146 (2008-03-29)
Reproducible techniques for the prefractionation of proteins prior to two-dimensional gel electrophoresis (2-DE) are essential for increasing the number of unique proteins that can be identified and assayed following biological experimentation. A simple and robust technique for separating highly soluble
Erik F Kot et al.
Langmuir : the ACS journal of surfaces and colloids, 34(11), 3426-3437 (2018-02-28)
Isotropic phospholipid bicelles are one of the most prospective membrane mimetics for the structural studies of membrane proteins in solution. Recent works provided an almost full set of data regarding the properties of isotropic bicelles; however, one major aspect of
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