16:0-14 Doxyl PC

Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.

16:0-14 Doxyl PC has been used:

• as a spin label for electron paramagnetic resonance (EPR) studies
• in labeling of small unilamellar vesicles (SUV) for electron spin resonance (ESR) studies
• in polarity profile studies of phospholipid membranes by high-frequency ESR

16:0-14 Doxyl phosphatidylcholine (PC) is used in the labeling of small unilamellar vesicles (SUVs) to probe the membrane depth from the hydrocarbon-water interface. n-Doxyl PC is incorporated in membranes for depth quenching measurements. It is used in fluorescence based vesicle quenching studies.

5 mL Clear Glass Sealed Ampule (810605C-1mg)

Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.

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