330003S
Avanti
E06 mAb-TopFluor®
E06 Mouse Monoclonal Antibody (IgM), TopFluor® labeled Anti-(Oxidized Phospholipid), 1X TBS (Tris Buffered Saline) with 1%BSA
Sign Into View Organizational & Contract Pricing
All Photos(1)
E06 mAb-TopFluor®, T15 antibody
Recommended Products
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
Assay
>95%
form
solution
packaging
pkg of 1 × 0.1 mL (polypropylene tube with screw cap (330003S-25ug))
manufacturer/tradename
Avanti Polar Lipids 330003S
concentration
0.25 mg/mL (330003S-25ug)
shipped in
dry ice
storage temp.
−70°C
General description
E06, an IgM mouse autoantibody is also called T15 derived from apolipoprotein E‐deficient mice.
The E06-TopFluor® is primarily use for direct ELISA, immune-fluorescence microscopy and flow cytometry. The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is verified for activity and provided with detailed protocols for the above applications. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
Application
E06 mAb-TopFluor® has been used:
- in the detection of oxidized phospholipids by immunocytochemical method in human primary skin fibroblasts
- as a component of blocking solution in competitive binding assay of oxidized phosphatidylcholine
- for the detection of oxidized phospholipids (OxPL) in the extracellular vesicle by flow cytometry
This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques.
Biochem/physiol Actions
E06 blocks the transient receptor potential cation channel subfamily V member 1 (TRPV1) channels.
Packaging
0.5 mL Polypropylene Tube with Screw Cap (330003S-25ug)
Preparation Note
For binding studies with the E06-TopFluor® antibody, 96-well Microfluor 1 black U bottom plates (Thermo 7005) were coated with 50μL of antigen (5μg/mL) in PBS containing 0.27 mM EDTA and overnight at 4°C. The wells were washed three times with PBS containing 0.27 mM EDTA. After washing, the plates were blocked with 3% BSA in PBS for 45 min at room temperature. 100μL aliquots of a serial dilution of the E06-TopFluor® antibody (0.78-400ng/well) in PBS containing EDTA and 1% BSA were added to each well and incubated 1hr at room temperature. The amount of IgM bound to each well was quantified with after 1hr incubation at room temperature with excitation (Ex) at 485 nm and emission (Em) recorded at 520 nm.
Legal Information
TopFluor is a registered trademark of Avanti Polar Lipids, Inc.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
含少量动物源组分生物产品
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Interferon regulatory factor 1-Rab27a regulated extracellular vesicles promote liver ischemia/reperfusion injury
Hepatology, 67(3), 1056-1070 (2018)
Fluorescence labeling of carbonylated lipids and proteins in cells using coumarin-hydrazide
Redox Biology, 5, 195-204 (2015)
Antinociception by the anti-oxidized phospholipid antibody E06
British Journal of Pharmacology, 175(14), 2940-2955 (2018)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service