131304P
Avanti
Glucosylceramide (Soy)
Avanti Polar Lipids
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Cerebroside (Soy); GlcCer(d18:2/16:0)
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description
Glucocerebrosides (Soy, 98%)
Assay
>99% (TLC)
form
powder
packaging
pkg of 1 × 10 mg (131304P-10mg)
pkg of 1 × 25 mg (131304P-25mg)
pkg of 1 × 5 mg (131304P-5mg)
manufacturer/tradename
Avanti Polar Lipids
lipid type
sphingolipids
shipped in
dry ice
storage temp.
−20°C
General description
Glucosylceramide (GluCer) is a major sphingolipid of plant tissue and, thus, abundant in nature and in dietary food sources. This study characterized the molecular species of GluCer from soybean and wheat by low-resolution, high-resolution and tandem mass spectrometry. Soybean GluCer was comprised primarily (>98%) of ceramide with 4,8-sphingadiene (d18:2(delta4,delta8)) and alpha- hydroxypalmitic acid (h16:0); the remainder had the same backbone with h18:0, h20:0, h22:0 and h24:0 fatty acids. Wheat GluCer had three major ceramide, d18:2(delta4,delta8) with h16:0, d18:1(delta8) with h16:0 and d18: 2(delta4,delta8) with h20:0, and smaller amounts of other homologs. These backbones differ from those of mammalian sphingolipids, which often have a delta4-double bond (but rarely a delta8-double bond), and have alpha-hydroxy fatty acids in only some cases.
Application
Glucosylceramide (Soy) has been used for the quantitative analysis of plant sphingolipid long-chain bases by gas chromatography-mass spectrometry (GC-MS). It has also been used for enzyme linked immunosorbent assay (ELISA) assay.
Glucosylceramide (Soy) has been used:
- in liposome preparation to test it effect on human α-synuclein aggregation
- as a long chain base source to test alkaline hydrolysis
- to test its immune modulatory effect on natural killer T lymphocytes and dendritic cells
Glucosylceramide (Soy) is suitable:
- for intraperitoneal/oral administration, to treat mice in order to the study the period as an anti-inflammatory model to prevent fibrosis induction
- for the preparation of glycolipids to study its effect on immune mediated colitis
- to coat the wells of microtiter plate and perform enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
The lipid backbones of mammalian GluCer (sphingosine, d18:1(delta4), and ceramide) induce cell death (apoptosis) and inhibit colon carcinogenesis, it is critical to know the structures of GluCer present in plants as a first step toward understanding this potential link between diet and cancer. Previously unexplained fragmentations that were diagnostic for the type of sphingoid base backbone (i.e. by homolytic cleavage of the doubly allylic C-6-C-7 bond to yield a stable distonic allylic radical cation and an allylic radical neutral) were also identified. Hence this method should be useful in the identification of double bonds in sphingolipids, and structure-function relationships between sphingolipids and colon carcinogenesis.†
Packaging
5 mL Amber Glass Screw Cap Vial (131304P-10mg)
5 mL Amber Glass Screw Cap Vial (131304P-25mg)
5 mL Amber Glass Screw Cap Vial (131304P-5mg)
WGK
WGK 3
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Detection of Antibody against Fungal Glucosylceramide in Immunocompromised Patients: A Potential New Diagnostic Approach for Cryptococcosis
Mycopathologia, 173(5-6), 419?425-419?425 (2012)
??Glucosylceramide: a novel method for enhancement of natural killer T lymphoycte plasticity in murine models of immune?mediated disorders
Gut, 56(1), 82?89-82?89 (2007)
Glucocerebroside treatment ameliorates ConA hepatitis by inhibition of NKT lymphocytes
American Journal of Physiology: Gastrointestinal and Liver Physiology, 289(5), G917-G925 (2005)
Amelioration of hepatic fibrosis via betaglucosylceramide-mediated immune modulation is associated with altered CD8 and NKT lymphocyte distribution
International Immunology, 19(8), 1021?1029-1021?1029 (2007)
Detection of antibody against fungal glucosylceramide in immunocompromised patients: a potential new diagnostic approach for cryptococcosis
Mycopathologia, 173(5-6), 419-425 (2012)
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