100600P
Avanti
E. coli Extract Polar
Avanti Research™ - A Croda Brand
Synonym(s):
E. coli Polar Lipid Extract
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About This Item
form
powder
packaging
pkg of 1 × 1 g (100600P-1g)
pkg of 1 × 100 mg (100600P-100mg)
pkg of 5 × 100 mg (100600P-500mg)
manufacturer/tradename
Avanti Research™ - A Croda Brand
lipid type
lipid extracts
shipped in
dry ice
storage temp.
−20°C
General description
This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.
Application
E. coli Extract Polar has been used for the preparation of lipid standards for validation purposes. It has also been used for determining the molar percentages of lipids.
E. coli Extract Polar has been used:
- in liposome preparation for spectroscopy studies using fluorescent membrane dye, laurdan
- in multilamellar vesicles generation
- as standard lipid material for tandem mass spectrometry analysis
E. coli Extract Polar is suitable: to prepare lipid standard material by dissolving in methanol to prepare small unilamellar vesicle to prepare liposomes (in Tris-hydrochloric acid (HCl)
Biochem/physiol Actions
E. coli Polar lipid extracts are useful for reconstituting membrane proteins. The phosphatidylethanolamine is the predominant lipid component of E. coli extract polar extract. The minor components include the phosphatidylglycerol (PG) and cardiolipin. These lipids are majorly derived from the leaflets of cytoplasmic membrane and inner leaflet of outer membrane. E. coli polar lipid extracts form bilayer by vesicle fusion.
Packaging
5 mL Clear Glass Sealed Ampule (100600P-100mg)
5 mL Clear Glass Sealed Ampule (100600P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100600P-1g)
Legal Information
Avanti Research is a trademark of Avanti Polar Lipids, LLC
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Assessing membrane fluidity and visualizing fluid membrane domains in bacteria using fluorescent membrane dyes
Wenzel M, et al.
Bio-protocol (2018)
Molecular model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers
Scheidelaar S, et al.
Biophysical Journal, 108(2), 279-290 (2015)
Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins
Methods in Cell Biology, 128, 223-241 (2015)
Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
Wenzel M, et al.
Bio-protocol, 8(20) (2018)
Emily Peterson et al.
Biochimica et biophysica acta, 1808(2), 516-521 (2010-10-26)
Amantadine-sensitive proton uptake by liposomes is currently the preferred method of demonstrating M2 functionality after reconstitution, to validate structural determination with techniques such as solid-state NMR. With strong driving forces (two decades each of both [K(+)] gradient-induced membrane potential and
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