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QBD10774

Sigma-Aldrich

NHS-dPEG®24-biotin

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Synonym(s):
Polyethylene glycol
Empirical Formula (Hill Notation):
C65H120N4O30S
Molecular Weight:
1469.72
UNSPSC Code:
12352200
PubChem Substance ID:
329823948
NACRES:
NA.22

Assay

>90%

form

solid or viscous liquid

reaction suitability

reaction type: Biotinylations
reactivity: amine reactive
reagent type: cross-linking reagent

polymer architecture

shape: linear
functionality: monofunctional

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(CCCCC1SCC(N2)C1NC2=O)NCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCC(ON3C(CCC3=O)=O)=O

Related Categories

General description

NHS-dPEG®24-biotin is a cross-linking reagent used in biotinylation reactions. The N-hydroxysuccinimidyl (NHS) moiety of NHS-dPEG®24-biotin forms a stable amide bond with free primary amines, such as the amines on the side chain of lysine. The biotin moiety is used in a variety of applications, including labeling, supramolecular building, affinity chromatography, pull-down assays, and the production of biotinylated antibodies.

Features and Benefits

NHS-dPEG®24-biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG®24-biotin compound has a long spacer of precise length (97.7 Å) between the biotin moiety and the labeled molecule. This dPEG® spacer imparts a high degree of water solubility to the biotin label. For biotinylated peptides and proteins, the dPEG® spacer dramatically reduces aggregation and precipitation of the modified molecule and reduces non-specific binding of the molecule, both of which are common problems with molecules that have been biotinylated with the widely used NHS-LC-biotin.

Legal Information

Products Protected under U.S. Patents # 7,888,536 B2
dPEG is a registered trademark of Quanta BioDesign

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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