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Safety Information

L033000

Sigma-Aldrich

BTT Activator

0.3 M

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Synonym(s):
5-(Benzylthio)-1H-tetrazole solution
Empirical Formula (Hill Notation):
C8H8N4S
CAS Number:
21871-47-6
Molecular Weight:
192.24
MDL number:
MFCD00068731
UNSPSC Code:
12352200
PubChem Substance ID:
329817353
NACRES:
NA.21

Quality Level

200

type

for DNA synthesis

product line

Proligo Reagents

storage condition

dry at room temperature

concentration

51.9-63.4 g/L (BTT content, titration)

color

clear

SMILES string

C(Sc1nnn[nH]1)c2ccccc2

InChI

1S/C8H8N4S/c1-2-4-7(5-3-1)6-13-8-9-11-12-10-8/h1-5H,6H2,(H,9,10,11,12)

InChI key

GXGKKIPUFAHZIZ-UHFFFAOYSA-N

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General description

Activator solution, containing powerful activators like 4,5-dicyanoimidazole(DCI), 5-(3,5-bis(trifluoromethyl)phenyl)-1H-tetrazole (Activator 42) or5-(Ethylthio)-1H-tetrazole (ETT) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.BTT Activator 0.3M is 5-(Benzylthio)-1H-tetrazole.

Preparation Note

Acetonitrile / 5-(Benzylthio)-1H-Tetrazole 100 / 6 (v/w)

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2

WGK

WGK 3

Flash Point(F)

41.0 °F

Flash Point(C)

5 °C

Regulatory Information

危险化学品

Certificates of Analysis (COA)

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