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928259

Sigma-Aldrich

Gold nanoparticles

40 nm diameter, azide functionalized, 1ml, OD 50, in water

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Synonym(s):
AuNP, GdNP, Gold NP
UNSPSC Code:
12141717
NACRES:
NA.22

Quality Level

100

description

Alkyne surface density: 1/nm2

concentration

in water

OD

50

core diam.

40 nm , coefficient of variance <12%

diameter

40 nm

λmax

530 nm

PDI

<0.200

functional group

azide

storage temp.

2-8°C

InChI

1S/Au

InChI key

PCHJSUWPFVWCPO-UHFFFAOYSA-N

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General description

Azide functionalized gold nanoparticles are suitable for covalent conjugation of any molecule with a terminal alkyne through click chemistry. These gold nanoparticles allow for rapid generation of gold conjugates for subsequent use in downstream assays.

Azide functionalized gold nanoparticles are more than 95% spherical and have uniform size distribution (CV <12%).

Application

Ideal for development of gold conjugates for use in applications such as blotting, lateral flow assays, microscopy, and transmission electron microscopy (TEM).

Storage and Stability

When stored for a long period of time gold nanoparticles may sediment at the bottom of the vial, which is especially prominent for larger particle sizes. Prior to use, re-suspend the sedimented particles by thorough mixing until a homogenous solution is obtained.

WGK

WGK 2

Regulatory Information

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Jennifer L Brennan et al.
Bioconjugate chemistry, 17(6), 1373-1375 (2006-11-16)
A simple and versatile method for the preparation of functional enzyme-gold nanoparticle conjugates using "click" chemistry has been developed. In a copper-catalyzed 1,2,3-triazole cycloaddition, an acetylene-functionalized Thermomyces lanuginosus lipase has been attached to azide-functionalized water-soluble gold nanoparticles under retention of
Vivek Poonthiyil et al.
Beilstein journal of organic chemistry, 14, 11-24 (2018-01-31)
Glycoscience, despite its myriad of challenges, promises to unravel the causes of, potential new detection methods for, and novel therapeutic strategies against, many disease states. In the last two decades, glyco-gold nanoparticles have emerged as one of several potential new
Monika Fischler et al.
Chemical communications (Cambridge, England), (2), 169-171 (2007-12-20)
We present a new type of azide-functionalized gold nanoparticle and their coupling to an alkyne-modified DNA duplex using the copper(I)-catalyzed Huisgen cycloaddition ('click chemistry'), resulting in a chain-like assembly of nanoparticles on the DNA template.

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