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Merck
CN

861049

Azure A chloride

certified by the BSC, Dye content 70 %

Synonym(s):

Methylene blue

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About This Item

Empirical Formula (Hill Notation):
C14H14ClN3S
CAS Number:
531-53-3
Molecular Weight:
291.80
EC Number:
208-510-7
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
329769437
MDL number:
MFCD00012112
Colour Index Number:
52005
Beilstein/REAXYS Number:
3922287

Key Documents

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Product Name

Azure A chloride, certified by the BSC, Dye content 70 %

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

SMILES string

NC1=CC(SC(C2=N3)=CC(C=C2)=[N+](C)C)=C3C=C1.[Cl-]

agency

certified by the BSC

form

powder

composition

Dye content, 70%

color

blue to very dark blue
very dark green

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL

λmax

633 nm

ε (extinction coefficient)

≥11000 at 242-246 nm in H2O at -1
≥26000 at 287-291 nm in H2O
≥34000 at 620-634 μm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Application

Azure A has been used to determine the amount of charged dextran sulfate in the nanoparticles for the study involving incorporation of heparin-binding proteins into preformed dextran sulfate-chitosan nanoparticles.
Certified for use in Lillie′s modified Nocht′s method and the NCCLS method for blood smears.

Biochem/physiol Actions

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBS1708
SHBR7981
SHBR7072
SHBS1277
SHBP7368

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How Romanowsky stains work and why they remain valuable - including a proposed universal Romanowsky staining mechanism and a rational troubleshooting scheme.
Horobin RW
Biotechnic & Histochemistry, 86, 36-51 (2011)
Heterochromatin in human male leukocytes.
Lima-de-Faria A and Reitalu J
The Journal of Cell Biology, 16.2, 315-322 (1963)
Quantification and characterization of glycosaminoglycans at the nanogram level by a combined azure A-silver staining in agarose gels.
van de Lest CH, et al.
Analytical Biochemistry, 221, 356-361 (1994)
Paula Zaman et al.
International journal of nanomedicine, 11, 6149-6159 (2016-12-07)
Incorporation of proteins into dextran sulfate (DS)-chitosan (CS) nanoparticles (DSCS NPs) is commonly performed using entrapment procedures, in which protein molecules are mixed with DS and CS until particle formation occurs. As DS is an analog of heparin, the authors
Q Chen et al.
Analytical and bioanalytical chemistry, 382(7), 1513-1519 (2005-06-14)
The interaction between azur A (AA) and hyaluronic acid (HA) at AA concentrations from 3.430 x 10(-5) to 8.575 x 10(-5) M and sodium chloride concentrations from 0 to 0.01 M was investigated spectrophotometrically at 620 nm at temperatures from
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