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Sigma-Aldrich

Auramine O

Dye content 85 %, certified by the Biological Stain Commission

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Synonym(s):
4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum
Empirical Formula (Hill Notation):
C17H21N3 · HCl
CAS Number:
2465-27-2
Molecular Weight:
303.83
Colour Index Number:
41000
Beilstein:
4030061
EC Number:
219-567-2
MDL number:
MFCD00012484
UNSPSC Code:
12171500
PubChem Substance ID:
24888527
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

200

form

powder or chunks

composition

Dye content, 85%

mp

>250 °C (dec.) (lit.)

solubility

95% ethanol: 1 mg/mL, clear to hazy, yellow to very deep yellow

λmax

370 nm
434 nm (2nd)

ε (extinction coefficient)

≥12000 at 249-255 nm in water at 0.005 g/L
≥15000 at 367-373 nm in water at 0.005 g/L
≥36000 at 429-435 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

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Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.

Biochem/physiol Actions

Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Suitability

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Signal Word

Warning

Hazard Statements

H302,H319,H351,H411

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Garcia LS
Practical Guide to Diagnostic Parasitology (2009)
Koneman's Color Atlas and Textbook of Diagnostic Microbiology (2006)
The Sigma-Aldrich Handbook of Stains, Dyes and Indicators,, 103-103 (1990)
The epidermal cell structure of the secondary pollen presenter in Vangueria infausta (Rubiaceae: Vanguerieae) suggests a functional association with protruding onci in pollen grains.
PLoS ONE, 9, e96405-e96405 (2014)
M Habtamu et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 16(3), 402-407 (2012-05-30)
Mawenzi Regional Hospital, northern Tanzania. To determine the value of light-emitting diode (LED) microscopy in diagnosing tuberculosis (TB) on bleach-treated and direct sputum smears. Sputum samples were collected from patients suspected of pulmonary TB who presented consecutively at the laboratory
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