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Merck
CN

860891

Bromocresol Purple sodium salt

indicator grade, Dye content 90 %

Synonym(s):

5′,5″-Dibromo-o-cresolsulfonphthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C21H15Br2NaO5S
CAS Number:
62625-30-3
Molecular Weight:
562.20
NACRES:
NA.47
PubChem Substance ID:
24888522
UNSPSC Code:
12171500
EC Number:
263-655-3
MDL number:
MFCD00148896

Key Documents

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Product Name

Bromocresol Purple sodium salt, indicator grade, Dye content 90 %

InChI

1S/C21H16Br2O5S.Na/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21;/h3-10,24-25H,1-2H3;/q;+1/p-1

InChI key

WNOIORUCCYNQPE-UHFFFAOYSA-M

SMILES string

[Na+].Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c([O-])c(Br)c4

grade

indicator grade

form

powder

composition

Dye content, 90%

technique(s)

titration: suitable

color

brown to very dark brown, to Very Dark Red

visual transition interval

5.2-6.8, yellow to purple

mp

255 °C (dec.) (lit.)

λmax

379 nm
585 nm (2nd)

ε (extinction coefficient)

≥47000 at 587-591 nm in 0.1 M NaOH
≥7000 at 375-381 nm in 0.1 M NaOH

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Bromocresol Purple is widely used as a vital stain to trace fluid diffusion in the lymphatic system. In addition, the dye is suitable to differentiate dead or live yeast cells. As a pH indicator, Bromocresol Purple is used in microbial culture media, both broth, and agar. In analytical experiments, e.g., to stain carbonic anhydrase isoenzyme on cellulose acetate electrophoresis membranes, bromocresol purple is used.

General description

Bromocresol Purple, also known as dibromo-o-cresolsulfonphthalein, is an acidic dye and belongs to the phthalein and sulphonphthalein dyes group. The dye color changes from yellow to violet at pH 5.2-6.8. The monosodium salt of the dye is 8% soluble in water and 2% in ethanol. Bromocresol Purple, under alkaline conditions, forms a hydrophilic, moderately sized dianion that is purple.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000521785
0000521785
0000509772
0000509772
0000505467

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Roland Krivanek et al.
Biophysical journal, 94(5), 1890-1903 (2007-11-13)
The energetics of the individual reaction steps in the catalytic cycle of photosynthetic water oxidation at the Mn(4)Ca complex of photosystem II (PSII) are of prime interest. We studied the electron transfer reactions in oxygen-evolving PSII membrane particles from spinach
Sabins RW
Handbook of Acid-Base Indicators (2007)
Senthilkumar Padmanaban et al.
Plant physiology, 144(1), 82-93 (2007-03-06)
Guard cell movement is induced by environmental and hormonal signals that cause changes in turgor through changes in uptake or release of solutes and water. Several transporters mediating these fluxes at the plasma membrane have been characterized; however, less is

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