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Safety Information

606871

Sigma-Aldrich

ISOGRO®-15N Powder -Growth Medium

98 atom % 15N

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About This Item

MDL number:
UNSPSC Code:
12352200
NACRES:
NA.12

isotopic purity

98 atom % 15N

Quality Level

technique(s)

bio NMR: suitable
protein expression: suitable

storage temp.

−20°C

Application

ISOGRO®-15N Powder -Growth Medium has been used:
  • for the production of 15N histone H2A for nuclear magnetic resonance (NMR) spectroscopy studies.
  • for the production of 15N-enriched protein kinase C and H3 histone for NMR studies.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Legal Information

ISOGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基

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Certificates of Analysis (COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the formulation of ISOGRO®-15N Powder?

    The nitrogen source is from green algae, not amino acids. The components are glucose, salts, water, nitrogen and carbon. The exact formulation is proprietary.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Expression and purification of the N-terminal regulatory domain of protein kinase C for biophysical studies
Cole TR and Igumenova TI
Protein Expression and Purification, 110(4), 14-21 (2015)
Mechanisms of ubiquitin-nucleosome recognition and regulation of 53BP1 chromatin recruitment by RNF168/169 and RAD18
Hu Q, et al.
Molecular Cell, 66(4), 473-487 (2017)
Alexandria N Richart et al.
The Journal of biological chemistry, 287(22), 18730-18737 (2012-04-12)
The chromoshadow domain (CSD) of heterochromatin protein 1 (HP1) was recently shown to contribute to chromatin binding and transcriptional regulation through interaction with histone H3. Here, we demonstrate the structural basis of this interaction for the CSD of HP1α. This
I Kilpelainen et al.
The Journal of biological chemistry, 275(18), 13564-13570 (2000-05-02)
Heparin-binding growth-associated molecule (HB-GAM) is an extracellular matrix-associated protein implicated in the development and plasticity of neuronal connections of brain. Binding to cell surface heparan sulfate is indispensable for the biological activity of HB-GAM. In the present paper we have
Xavier Hanoulle et al.
The Journal of biological chemistry, 282(47), 34148-34158 (2007-09-15)
The chemotaxis and integrin-mediated adhesion of T lymphocytes triggered by secreted cyclophilin B (CypB) depend on interactions with both cell surface heparan sulfate proteoglycans (HSPG) and the extracellular domain of the CD147 membrane receptor. Here, we use NMR spectroscopy to

Articles

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.

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