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Sigma-Aldrich

Ammonium-15N chloride

≥98 atom % 15N, ≥99% (CP)

Synonym(s):

15N Labeled ammonium chloride

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About This Item

Linear Formula:
15NH4Cl
CAS Number:
39466-62-1
Molecular Weight:
54.48
MDL number:
MFCD00075417
UNSPSC Code:
12142200
PubChem Substance ID:
24858029
NACRES:
NA.12

isotopic purity

≥98 atom % 15N

Quality Level

200

Assay

≥99% (CP)

technique(s)

bio NMR: suitable
protein expression: suitable

mp

340 °C (subl.) (lit.)

mass shift

M+1

SMILES string

[15NH4+].[Cl-]

InChI

1S/ClH.H3N/h1H;1H3/i;1+1

InChI key

NLXLAEXVIDQMFP-IEOVAKBOSA-N

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Application

Ammonium-15N chloride has been used:
  • as a medium supplement for the incorporation of 15N in sporulation protein, CsfB, for NMR spectroscopy
  • in assaying nitrogen loss in marine sediment
  • for the incorporation of 15N in apolipoprotein A-I for mass spectrum analysis

Biochem/physiol Actions

Ammonium chloride is a component of the nitrogen cycle. Excess of ammonium chloride depletes ATP in astrocytes. Ammonium chloride inhibits the acidification in endosome-lysosome system. It is a hemolysing agent used in the lysis of erythrocytes. Ammonium chloride administration promotes creatinine and urea clearance in rat.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The Journal of Organic Chemistry, 46, 4296-4296 (1981)
Characterization of homodimer interfaces with cross-linking mass spectrometry and isotopically labeled proteins
Lima DB, et al.
Nature Protocols, 13(3), 431-431 (2018)
Brianna J Klein et al.
Structure (London, England : 1993), 28(1), 105-110 (2019-11-13)
The assembly of human histone acetyltransferase MOZ/MORF complexes relies on the scaffolding bromodomain plant homeodomain (PHD) finger 1 (BRPF1) subunit. The PHD-zinc-knuckle-PHD module of BRPF1 (BRPF1PZP) has been shown to associate with the histone H3 tail and DNA; however, the
Erythrocyte lysis in isotonic solution of ammonium chloride: Theoretical modeling and experimental verification
Chernyshev AV, et al.
Journal of Theoretical Biology, 251(1), 93-107 (2008)
Journal of the Chemical Society. Chemical Communications, 62-62 (1982)
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