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235474

Sigma-Aldrich

Acridine Orange base

Dye content 75 %

Synonym(s):

3,6-Bis(dimethylamino)acridine, Solvent Orange 15

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About This Item

Empirical Formula (Hill Notation):
C17H19N3
CAS Number:
494-38-2
Molecular Weight:
265.35
Colour Index Number:
46005
MDL number:
MFCD00005029
UNSPSC Code:
12171500
PubChem Substance ID:
24854106
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 75%

mp

165 °C (dec.) (lit.)

solubility

1 M HCl: 1 mg/mL, clear

λmax

488 nm

ε (extinction coefficient)

≥14000 at 227-233 nm
≥20000 at 289-295 nm
≥35000 at 486-492 nm
≥40000 at 266-272 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI

1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key

DPKHZNPWBDQZCN-UHFFFAOYSA-N

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General description

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye.

Application

Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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