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Sigma-Aldrich

Direct Blue 71

Dye content 50 %

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Empirical Formula (Hill Notation):
C40H23N7O13S4 · 4Na
CAS Number:
4399-55-7
Molecular Weight:
1029.87
Colour Index Number:
34140
EC Number:
224-531-4
MDL number:
MFCD00003971
UNSPSC Code:
12171500
PubChem Substance ID:
24852733
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 50%

λmax

594 nm

ε (extinction coefficient)

≥15000 at 283-289 nm in water at 0.01 g/L
≥30000 at 582-588 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Nc1ccc2c(O)c(N=Nc3ccc(N=Nc4ccc(N=Nc5cc(c6cccc(c6c5)S([O-])(=O)=O)S([O-])(=O)=O)c7ccccc47)c8ccc(cc38)S([O-])(=O)=O)c(cc2c1)S([O-])(=O)=O

InChI

1S/C40H27N7O13S4.4Na/c41-22-8-10-25-21(16-22)17-38(64(58,59)60)39(40(25)48)47-46-35-15-14-34(28-11-9-24(20-30(28)35)61(49,50)51)45-44-33-13-12-32(26-4-1-2-5-27(26)33)43-42-23-18-31-29(37(19-23)63(55,56)57)6-3-7-36(31)62(52,53)54;;;;/h1-20,48H,41H2,(H,49,50,51)(H,52,53,54)(H,55,56,57)(H,58,59,60);;;;/q;4*+1/p-4

InChI key

QTTDXDAWQMDLOF-UHFFFAOYSA-J

Related Categories

General description

Direct blue 71 is an azo dye with three azo bonds. It is useful to stain membrane-immobilized antibodies and proteins for the investigation of antigen-antibody interactions.

Application

Direct blue 71 has been used to stain sliced tissue sections mounted on the PVDF for in situ proteome analysis, and to stain the electroblotted membranes and sections of the brain to visualize the structure. Direct blue staining is also suitable for the assessment of protein binding and qualitative validation of protein concentration.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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