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Sigma-Aldrich

Celestine blue

Dye content 80 %

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Synonym(s):
Mordant Blue 14
Empirical Formula (Hill Notation):
C17H18ClN3O4
CAS Number:
1562-90-9
Molecular Weight:
363.80
Colour Index Number:
51050
Beilstein:
3830911
EC Number:
216-346-2
MDL number:
MFCD00011927
UNSPSC Code:
12171500
PubChem Substance ID:
24852395
NACRES:
NA.47

form

powder

composition

Dye content, 80%

mp

227-230 °C

solubility

water: 0.1%

λmax

642 nm

ε (extinction coefficient)

≥29000 at 640-650 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)c(O)c(O)cc3C(N)=O

InChI

1S/C17H17N3O4.ClH/c1-3-20(4-2)9-5-6-11-13(7-9)24-16-14(19-11)10(17(18)23)8-12(21)15(16)22;/h5-8H,3-4H2,1-2H3,(H3-,18,19,21,22,23);1H

InChI key

VXAFJUDTZSMEFN-UHFFFAOYSA-N

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Application

May be substituted for hematoxylin in hematoxylin and eosin procedures.

Biochem/physiol Actions

Celestine blue (CB) is an oxazine dye and is used in combination with the alum hematoxylin, as a nuclear stain. It gives good nuclear definition and is resistant to strong acid dyes. Celestine blue is commonly used for routine staining of fixed sections. It has been used as a new electroactive indicator in DNA biosensors.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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H Troyer et al.
Stain technology, 56(1), 39-43 (1981-01-01)
A staining procedure is described for use with glycol methacrylate embedded tissue sections which does not stain the plastic embedment or remove the sections from the glass slides. The basic dye is celestine blue B. It is prepared by treating
A Rami et al.
Brain research, 866(1-2), 299-312 (2000-05-29)
The differentiated cells seem to share the ability to induce their own death by the activation of an internally encoded suicide program. When activated, this suicide program initiates a characteristic form of cell death called apoptosis. A central challenge in
J Yang et al.
American journal of clinical pathology, 65(5), 715-718 (1976-05-01)
The use of an iron alum lake of celestine blue B in acetic acid as an alternative to hematoxylin in the routine staining of fecal smears and tissue sections for Protozoa is presented.
Goodwill Trading Co
Histophatologic Techniques (2006)
Celestine blue as a new indicator in
electrochemical DNA biosensors
Asl E H
Science China: Chemistry (2015)
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