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202096

Sigma-Aldrich

New Methylene Blue N zinc chloride double salt

Dye content 90 %

Synonym(s):

Basic blue 24, Methylene blue N

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About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S · 0.5ZnCl2
CAS Number:
6586-05-6
Molecular Weight:
416.05
Colour Index Number:
52030
EC Number:
229-516-6
MDL number:
MFCD00078135
UNSPSC Code:
12171500
PubChem Substance ID:
24852036
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 90%

mp

>240 °C (dec.) (lit.)

λmax

591 nm
630 nm (2nd)

ε (extinction coefficient)

≥16000 at 242-248 nm in H2O
≥28000 at 284-290 nm in H2O
≥36000 at 588-594 nm in H2O
≥44000 at 629-635 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].Cl[Zn]Cl.CCNc1cc2[s+]c3cc(NCC)c(C)cc3nc2cc1C.CCNc4cc5[s+]c6cc(NCC)c(C)cc6nc5cc4C

InChI

1S/2C18H22N3S.4ClH.Zn/c2*1-5-19-13-9-17-15(7-11(13)3)21-16-8-12(4)14(20-6-2)10-18(16)22-17;;;;;/h2*7-10,19-20H,5-6H2,1-4H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

MCBJJSJOIVJYQY-UHFFFAOYSA-J

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Application

New Methylene Blue N zinc chloride double salt has been used as a photosensitizer for the in vitro photodynamic inactivation of plant-pathogenic fungi Colletotrichum acutatum and Colletotrichum gloeosporioides. It has also been used to determine the in vitro susceptibilities of Trichophyton mentagrophytes and Trichophyton rubrum microconidia to photodynamic antimicrobial chemotherapy (PACT).

Biochem/physiol Actions

New Methylene Blue N zinc chloride double salt also known as methylene blue N is an azo dye. It can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living, young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N zinc chloride double salt helps in counting of reticulocytes (erythrocytes containing RNA).

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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PHOTODEGRADATION OF NEW METHYLENE BLUE N IN
AQUEOUS SOLUTION USING ZINC OXIDE AND
TITANIUM DIOXIDE AS CATALYST
ALI R
Jurnal Teknologi, 45, 31-42 (2006)
In Vitro Photodynamic Inactivation of Plant-Pathogenic Fungi Colletotrichum acutatum and Colletotrichum gloeosporioides with Novel Phenothiazinium Photosensitizers
de Menezes HD
Applied and Environmental Microbiology, 80, 1623-1632 (2014)
Susceptibilities of the dermatophytes Trichophyton mentagrophytes and T. rubrum microconidia to photodynamic antimicrobial chemotherapy with novel phenothiazinium photosensitizers and red light
Rodrigues GB
Journal of Photochemistry and Photobiology. B, Biology, 89-94 (2012)
Mary Turgeon
Linne & Ringsrud's Clinical Laboratory Science (2011)
Gabriela Braga Rodrigues et al.
Journal of photochemistry and photobiology. B, Biology, 209, 111942-111942 (2020-07-06)
Antimicrobial photodynamic treatment (APDT) has emerged as an effective therapy against pathogenic fungi with both acquired and intrinsic resistance to commonly used antifungal agents. Success of APDT depends on the availability of effective photosensitizers capable of acting on different fungal

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